Back to Search
Start Over
Characteristic cellular composition of germinal centers.
Characteristic cellular composition of germinal centers.
- Source :
-
Comparative immunology, microbiology and infectious diseases [Comp Immunol Microbiol Infect Dis] 2009 Sep; Vol. 32 (5), pp. 419-28. Date of Electronic Publication: 2008 Mar 04. - Publication Year :
- 2009
-
Abstract
- We established the method of isolating individually encapsulated germinal centers (GCs) from immunized spleen and analyzed single cell suspension of GCs by flowcytometry. In GCs, the high frequency of sIgG+ cells (29%) and sIgA+ cells (5%) was detected. Two-color flowcytometry analysis showed that GCs contained 27% of sIgM-IgG+ cells, in which isotype switch from IgM to IgG had occurred, and 5% of Bu1-IgG+ cells, which were differentiating into plasma cells. On the other hand, sIgM-IgG+ and Bu1-IgG+ cells were not detected in the bursa, which contained 95% of B cells and only 1% of T cells. CD4+ but not CD8+ T cells were detected in the light zone of GCs and these CD4+ T cells are supposed to play a key role in isotype switching and differentiation into plasma cells in GCs. These results clearly demonstrate that GCs provide a site for isotype switching and differentiation into plasma cells.
- Subjects :
- Animals
B-Lymphocyte Subsets metabolism
Chickens immunology
Germinal Center cytology
Germinal Center metabolism
Immunoglobulins immunology
Immunoglobulins metabolism
Plasma Cells immunology
Plasma Cells metabolism
Spleen immunology
Spleen metabolism
T-Lymphocyte Subsets metabolism
B-Lymphocyte Subsets immunology
Cell Differentiation immunology
Germinal Center immunology
Immunoglobulin Class Switching
T-Lymphocyte Subsets immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1878-1667
- Volume :
- 32
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Comparative immunology, microbiology and infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 18291526
- Full Text :
- https://doi.org/10.1016/j.cimid.2007.12.002