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Characteristic cellular composition of germinal centers.

Characteristic cellular composition of germinal centers.

Authors :
Imamura K
Yasuda M
Riwar B
Inui S
Ekino S
Source :
Comparative immunology, microbiology and infectious diseases [Comp Immunol Microbiol Infect Dis] 2009 Sep; Vol. 32 (5), pp. 419-28. Date of Electronic Publication: 2008 Mar 04.
Publication Year :
2009

Abstract

We established the method of isolating individually encapsulated germinal centers (GCs) from immunized spleen and analyzed single cell suspension of GCs by flowcytometry. In GCs, the high frequency of sIgG+ cells (29%) and sIgA+ cells (5%) was detected. Two-color flowcytometry analysis showed that GCs contained 27% of sIgM-IgG+ cells, in which isotype switch from IgM to IgG had occurred, and 5% of Bu1-IgG+ cells, which were differentiating into plasma cells. On the other hand, sIgM-IgG+ and Bu1-IgG+ cells were not detected in the bursa, which contained 95% of B cells and only 1% of T cells. CD4+ but not CD8+ T cells were detected in the light zone of GCs and these CD4+ T cells are supposed to play a key role in isotype switching and differentiation into plasma cells in GCs. These results clearly demonstrate that GCs provide a site for isotype switching and differentiation into plasma cells.

Details

Language :
English
ISSN :
1878-1667
Volume :
32
Issue :
5
Database :
MEDLINE
Journal :
Comparative immunology, microbiology and infectious diseases
Publication Type :
Academic Journal
Accession number :
18291526
Full Text :
https://doi.org/10.1016/j.cimid.2007.12.002