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Ste20-related protein kinase LOSK (SLK) controls microtubule radial array in interphase.
- Source :
-
Molecular biology of the cell [Mol Biol Cell] 2008 May; Vol. 19 (5), pp. 1952-61. Date of Electronic Publication: 2008 Feb 20. - Publication Year :
- 2008
-
Abstract
- Interphase microtubules are organized into a radial array with centrosome in the center. This organization is a subject of cellular regulation that can be driven by protein phosphorylation. Only few protein kinases that regulate microtubule array in interphase cells have been described. Ste20-like protein kinase LOSK (SLK) was identified as a microtubule and centrosome-associated protein. In this study we have shown that the inhibition of LOSK activity by dominant-negative mutant K63R-DeltaT or by LOSK depletion with RNAi leads to unfocused microtubule arrangement. Microtubule disorganization is prominent in Vero, CV-1, and CHO-K1 cells but less distinct in HeLa cells. The effect is a result neither of microtubule stabilization nor of centrosome disruption. In cells with suppressed LOSK activity centrosomes are unable to anchor or to cap microtubules, though they keep nucleating microtubules. These centrosomes are depleted of dynactin. Vero cells overexpressing K63R-DeltaT have normal dynactin "comets" at microtubule ends and unaltered morphology of Golgi complex but are unable to polarize it at the wound edge. We conclude that protein kinase LOSK is required for radial microtubule organization and for the proper localization of Golgi complex in various cell types.
- Subjects :
- Animals
Catalytic Domain
Cell Line
Cell Polarity
Centrosome enzymology
Diffusion
Genes, Dominant
Golgi Apparatus enzymology
Humans
Mutant Proteins metabolism
Peptide Fragments metabolism
Protein Binding
Protein Serine-Threonine Kinases antagonists & inhibitors
Protein Serine-Threonine Kinases chemistry
Protein Serine-Threonine Kinases deficiency
Protein Transport
RNA Interference
Interphase
Microtubules enzymology
Protein Serine-Threonine Kinases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1939-4586
- Volume :
- 19
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Molecular biology of the cell
- Publication Type :
- Academic Journal
- Accession number :
- 18287541
- Full Text :
- https://doi.org/10.1091/mbc.E06-12-1156