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Mouse and human indoleamine 2,3-dioxygenase display some distinct biochemical and structural properties.
- Source :
-
Amino acids [Amino Acids] 2009 Jan; Vol. 36 (1), pp. 99-106. Date of Electronic Publication: 2008 Feb 15. - Publication Year :
- 2009
-
Abstract
- The hemoprotein indoleamine 2,3-dioxygenase (IDO) is the first and rate-limiting enzyme in the most significant pathway for mammalian tryptophan metabolism. It has received considerable attention in recent years, particularly due to its dual role in immunity and the pathogenesis of many diseases. Reported here are differences and similarities between biochemical behaviour and structural features of recombinant human IDO and recombinant mouse IDO. Significant differences were observed in the conversion of substrates and pH stability. Differences in inhibitor potency and thermal stability were also noted. Secondary structural features were broadly similar but variation between species was apparent, particularly in the alpha-helix portion of the enzymes. With mouse models substituting for human diseases, the differences between mouse and human IDO must be recognised before applying experimental findings from one system to the next.
- Subjects :
- Amino Acid Sequence
Animals
Circular Dichroism
Enzyme Stability
Humans
Hydrogen-Ion Concentration
Indoleamine-Pyrrole 2,3,-Dioxygenase genetics
Indoleamine-Pyrrole 2,3,-Dioxygenase isolation & purification
Kinetics
Kynurenine chemistry
Kynurenine metabolism
Mice
Molecular Sequence Data
Protein Folding
Protein Structure, Secondary
Recombinant Proteins chemistry
Recombinant Proteins genetics
Recombinant Proteins isolation & purification
Recombinant Proteins metabolism
Sequence Alignment
Temperature
Indoleamine-Pyrrole 2,3,-Dioxygenase chemistry
Indoleamine-Pyrrole 2,3,-Dioxygenase metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1438-2199
- Volume :
- 36
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Amino acids
- Publication Type :
- Academic Journal
- Accession number :
- 18274832
- Full Text :
- https://doi.org/10.1007/s00726-008-0037-6