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Molecular and biochemical characterization of Toxoplasma gondii beta-hydroxyacyl-acyl carrier protein dehydratase (FABZ).
- Source :
-
Parasitology research [Parasitol Res] 2008 May; Vol. 102 (6), pp. 1301-9. Date of Electronic Publication: 2008 Feb 15. - Publication Year :
- 2008
-
Abstract
- Toxoplasma gondii, unlike its mammalian host, utilizes a type II fatty acid biosynthesis pathway in which the steps of fatty acid biosynthesis are catalyzed by independent enzymes. Due to this difference, the enzymes of this pathway are good targets for the development of new therapeutic drugs directed against toxoplasmosis. In this report, we show by using reverse transcription-polymerase chain reaction analysis that beta-Hydroxyacyl-acyl carrier protein dehydratase (TgFABZ) is expressed both in tachyzoites and bradyzoites. Indirect immunofluorescence antibody test further shows the localization of TgFABZ protein in the apicoplast of both tachyzoites and bradyzoites. Enzyme dynamic analysis shows that the purified recombinant TgFABZ protein is soluble and active. The Km value of the enzyme for its substrate analog crotonoyl-CoA was estimated to be 82.57 +/- 10 microM.
- Subjects :
- Acyl Coenzyme A metabolism
Animals
Antigens, Protozoan analysis
Female
Fluorescent Antibody Technique, Indirect
Gene Expression Profiling
Hydro-Lyases isolation & purification
Kinetics
Mice
Protozoan Proteins isolation & purification
Recombinant Proteins genetics
Recombinant Proteins isolation & purification
Recombinant Proteins metabolism
Reverse Transcriptase Polymerase Chain Reaction methods
Toxoplasma chemistry
Hydro-Lyases genetics
Hydro-Lyases metabolism
Protozoan Proteins genetics
Protozoan Proteins metabolism
Toxoplasma enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 0932-0113
- Volume :
- 102
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Parasitology research
- Publication Type :
- Academic Journal
- Accession number :
- 18274777
- Full Text :
- https://doi.org/10.1007/s00436-008-0909-4