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Co-option of a default secretory pathway for plant immune responses.
- Source :
-
Nature [Nature] 2008 Feb 14; Vol. 451 (7180), pp. 835-40. - Publication Year :
- 2008
-
Abstract
- Cell-autonomous immunity is widespread in plant-fungus interactions and terminates fungal pathogenesis either at the cell surface or after pathogen entry. Although post-invasive resistance responses typically coincide with a self-contained cell death of plant cells undergoing attack by parasites, these cells survive pre-invasive defence. Mutational analysis in Arabidopsis identified PEN1 syntaxin as one component of two pre-invasive resistance pathways against ascomycete powdery mildew fungi. Here we show that plasma-membrane-resident PEN1 promiscuously forms SDS-resistant soluble N-ethylmaleimide sensitive factor attachment protein receptor (SNARE) complexes together with the SNAP33 adaptor and a subset of vesicle-associated membrane proteins (VAMPs). PEN1-dependent disease resistance acts in vivo mainly through two functionally redundant VAMP72 subfamily members, VAMP721 and VAMP722. Unexpectedly, the same two VAMP proteins also operate redundantly in a default secretory pathway, suggesting dual functions in separate biological processes owing to evolutionary co-option of the default pathway for plant immunity. The disease resistance function of the secretory PEN1-SNAP33-VAMP721/722 complex and the pathogen-induced subcellular dynamics of its components are mechanistically reminiscent of immunological synapse formation in vertebrates, enabling execution of immune responses through focal secretion.
- Subjects :
- ATP-Binding Cassette Transporters genetics
ATP-Binding Cassette Transporters metabolism
Arabidopsis genetics
Arabidopsis Proteins genetics
Ascomycota physiology
N-Glycosyl Hydrolases genetics
N-Glycosyl Hydrolases metabolism
Qa-SNARE Proteins genetics
Qa-SNARE Proteins metabolism
SNARE Proteins genetics
SNARE Proteins metabolism
Arabidopsis immunology
Arabidopsis microbiology
Arabidopsis Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1476-4687
- Volume :
- 451
- Issue :
- 7180
- Database :
- MEDLINE
- Journal :
- Nature
- Publication Type :
- Academic Journal
- Accession number :
- 18273019
- Full Text :
- https://doi.org/10.1038/nature06545