Back to Search
Start Over
Systematic determination of differential gene expression in the primate corpus luteum during the luteal phase of the menstrual cycle.
- Source :
-
Molecular endocrinology (Baltimore, Md.) [Mol Endocrinol] 2008 May; Vol. 22 (5), pp. 1260-73. Date of Electronic Publication: 2008 Feb 07. - Publication Year :
- 2008
-
Abstract
- The molecular and cellular processes required for development, function, and regression of the primate corpus luteum (CL) are poorly defined. We hypothesized that there are dynamic changes in gene expression occurring during the CL life span, which represent proteins and pathways critical to its regulation. Therefore, a genomic approach was utilized to systematically identify differentially expressed genes in the rhesus macaque CL during the luteal phase of natural menstrual cycles. CL were collected between d 3-5 (early stage), d 7-8 (mid), d 10-12 (mid-late), d 14-16 (late), or d 18-19 (very-late) after the midcycle LH surge. From the early through very-late stages, 3234 transcripts were differentially expressed, with 879 occurring from the early through late stages that encompass the processes of luteinization, maintenance, and functional regression. To characterize gene changes most relevant to these processes, ontology analysis was performed using the list of 879 differentially expressed transcripts. Four main groups of related genes were identified with relevance to luteal physiology including: 1) immune function; 2) hormone and growth factor signaling; 3) steroidogenesis; and 4) prostaglandin biosynthesis, metabolism, and signaling. A subset of genes representing each of the four major categories was selected for validation of microarray results by quantitative real-time PCR. Results in mRNA levels were similar between the two methodologies for 17 of 18 genes. Additionally, protein levels for three genes were determined by Western blot analysis to parallel mRNA levels. This database will facilitate the identification of many novel or previously underappreciated pathways that regulate the structure and function of the primate CL.
- Subjects :
- Animals
Blotting, Western
Female
Oligonucleotide Array Sequence Analysis
RNA, Messenger genetics
RNA, Messenger metabolism
Receptors, Prolactin genetics
Receptors, Prolactin metabolism
Reverse Transcriptase Polymerase Chain Reaction
Corpus Luteum metabolism
Gene Expression Profiling methods
Luteal Phase
Macaca mulatta genetics
Menstrual Cycle
Subjects
Details
- Language :
- English
- ISSN :
- 0888-8809
- Volume :
- 22
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Molecular endocrinology (Baltimore, Md.)
- Publication Type :
- Academic Journal
- Accession number :
- 18258683
- Full Text :
- https://doi.org/10.1210/me.2007-0484