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Similarity of responses of cultured Sertoli cells to cholera toxin and FSH.
- Source :
-
Molecular and cellular endocrinology [Mol Cell Endocrinol] 1976 Aug-Sep; Vol. 5 (3-4), pp. 289-94. - Publication Year :
- 1976
-
Abstract
- The effects of cholera toxin on the responses of cultured Sertoli cells were compared with those elicited by follicle-stimulating hormone (FSH), and N6O2'-dibutyryl-3',5'-cyclic AMP (bu2cAMP). Addition of FSH or cholera toxin increased cAMP levels. Subsequently there was greater rates of conversion of testosterone to 17beta-estradiol, formation of androgen-binding protein (ABP), and incorporation of [3H]thymidine into DNA by Sertoli cells prepared from testes of immature rats and cultured in the presence of either FSH or cholera toxin. Addition of bu2-cAMP also resulted in enhanced rates of formation of ABP, synthesis of 17beta-estradiol and synthesis of DNA. Cholera toxin and bu2-cAMP elicited changes in morphology of cultured Sertoli cells indistinguishable from those following FSH addition. It is concluded that elevated intracellular cAMP levels can duplicate known actions of FSH on cultured Sertoli cells, but the possible obligatory role of cAMP in mediating FSH actions remains to be evaluated.
- Subjects :
- Animals
Bucladesine pharmacology
Cells, Cultured
Cyclic AMP metabolism
DNA Replication drug effects
Estradiol biosynthesis
Male
Rats
Sertoli Cells drug effects
Testosterone metabolism
Follicle Stimulating Hormone pharmacology
Sertoli Cells metabolism
Toxins, Biological pharmacology
Vibrio cholerae
Subjects
Details
- Language :
- English
- ISSN :
- 0303-7207
- Volume :
- 5
- Issue :
- 3-4
- Database :
- MEDLINE
- Journal :
- Molecular and cellular endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 182580
- Full Text :
- https://doi.org/10.1016/0303-7207(76)90090-3