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Biological hydrogen production by immobilized cells of Clostridium tyrobutyricum JM1 isolated from a food waste treatment process.

Biological hydrogen production by immobilized cells of Clostridium tyrobutyricum JM1 isolated from a food waste treatment process.

Authors :
Jo JH
Lee DS
Park D
Park JM
Source :
Bioresource technology [Bioresour Technol] 2008 Sep; Vol. 99 (14), pp. 6666-72. Date of Electronic Publication: 2008 Jan 14.
Publication Year :
2008

Abstract

A fermentative hydrogen-producing bacterium, Clostridium tyrobutyricum JM1, was isolated from a food waste treating process using 16S rRNA gene sequencing and amplified ribosomal DNA restriction analysis (ARDRA). A fixed-bed bioreactor packed with polyurethane foam as support matrix for the growth of the isolate was operated at different hydraulic retention time (HRT) to evaluate its performance for hydrogen production. The reactor achieved the maximal hydrogen production rate of 7.2 l H(2)l(-1)d(-1) at 2h HRT, where hydrogen content in biogas was 50.0%, and substrate conversion efficiency was 97.4%. The maximum hydrogen yield was 223 ml (g-hexose)(-1) with an influent glucose concentration of 5 g l(-1). Therefore, the immobilized reactor using C. tyrobutyricum JM1 was an effective and stable system for continuous hydrogen production.

Details

Language :
English
ISSN :
0960-8524
Volume :
99
Issue :
14
Database :
MEDLINE
Journal :
Bioresource technology
Publication Type :
Academic Journal
Accession number :
18248983
Full Text :
https://doi.org/10.1016/j.biortech.2007.11.067