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Use of site occupancy models to estimate prevalence of Myxobolus cerebralis infection in trout.
- Source :
-
Journal of aquatic animal health [J Aquat Anim Health] 2007 Mar; Vol. 19 (1), pp. 8-13. - Publication Year :
- 2007
-
Abstract
- Empirical estimates of pathogen prevalence in samples of fish may underestimate true prevalence because available detection techniques are incapable of perfect detection. Trout of several species were collected from enzootic (Myxobolus cerebralis, causative agent in whirling disease) habitats, and individual fish were examined for presence of the parasite two or six times by one of four methods: pepsin-trypsin digest (brown trout Salmo trutta), plankton centrifuge (brown trout), polymerase chain reaction (rainbow trout Oncorhynchus mykiss), or histopathology (brook trout Salvelinus fontinalis). The presence-absence data were modeled for prevalence of infection (psi) and probability of detection (p) of the parasite via occupancy models that accounted for imperfect detection of the organism. Based on estimates from the most-supported model for comparison, two myxospore concentration methods underestimated prevalence by about 12% for whole-head results and 34% for the expected value of half-head analysis. Polymerase chain reaction and histopathology gave virtually the same prevalence estimates for whole-head results as the best models but underestimated prevalence by about 6% and 12%, respectively, for the expected value of half-head analysis. The probability of detecting the parasite in a single survey of a fish head, conditional on the parasite's presence, was 0.66 for myxospore concentration methods, 0.81 for histopathology, and 1.0 (left halves) or 0.89 (right halves) for polymerase chain reaction. The occupancy models used in this study may be extended to large-scale monitoring of M. cerebralis to estimate expansion or contraction of the parasite's range over time.
- Subjects :
- Animals
Diagnosis, Differential
Fish Diseases diagnosis
Fish Diseases pathology
Head parasitology
Polymerase Chain Reaction veterinary
Predictive Value of Tests
Prevalence
Probability
Protozoan Infections, Animal diagnosis
Protozoan Infections, Animal pathology
Sensitivity and Specificity
Eukaryota isolation & purification
Fish Diseases epidemiology
Protozoan Infections, Animal epidemiology
Trout parasitology
Subjects
Details
- Language :
- English
- ISSN :
- 0899-7659
- Volume :
- 19
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of aquatic animal health
- Publication Type :
- Academic Journal
- Accession number :
- 18236627
- Full Text :
- https://doi.org/10.1577/H06-016.1