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Purification of SPARC/osteonectin.

Authors :
Sage EH
Source :
Current protocols in cell biology [Curr Protoc Cell Biol] 2003 Feb; Vol. Chapter 10, pp. Unit 10.11.
Publication Year :
2003

Abstract

SPARC is a matricellular protein that regulates cell adhesion, extracelluolar matrix production, growth factor activity and cell cycle. This unit describes the purification of SPARC, also termed osteonectin and BM/40, from cultured mammalian cells. Additional information is presented on the purification of recombinant SPARC (rSPARC) from E. coli and from Sf9 cells, as well as its isolation from blood platelets. Assays for the activity of SPARC, de-adhesion and inhibition of cellular proliferation in vitro, are described. The expression of SPARC during remodeling and repair tissue in response to injury identifies it as a therapeutic target for the treatment of fibrotic disease, certain cancers and other disorders in which regulation of angiogenesis is a key factor.

Details

Language :
English
ISSN :
1934-2616
Volume :
Chapter 10
Database :
MEDLINE
Journal :
Current protocols in cell biology
Publication Type :
Academic Journal
Accession number :
18228415
Full Text :
https://doi.org/10.1002/0471143030.cb1011s17