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Interaction trap/two-hybrid system to identify interacting proteins.

Authors :
Golemis EA
Serebriiskii I
Finley RL Jr
Kolonin MG
Gyuris J
Brent R
Source :
Current protocols in cell biology [Curr Protoc Cell Biol] 2001 May; Vol. Chapter 17, pp. Unit 17.3.
Publication Year :
2001

Abstract

This unit presents protocols designed to detect interacting proteins. Using yeast as a "test tube" and transcriptional activation of a reporter system, interacting proteins can be identified. The system can also be used to test complex formation for proteins for which there exists a reason to expect interaction.

Details

Language :
English
ISSN :
1934-2616
Volume :
Chapter 17
Database :
MEDLINE
Journal :
Current protocols in cell biology
Publication Type :
Academic Journal
Accession number :
18228339
Full Text :
https://doi.org/10.1002/0471143030.cb1703s08