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Interaction trap/two-hybrid system to identify interacting proteins.
- Source :
-
Current protocols in cell biology [Curr Protoc Cell Biol] 2001 May; Vol. Chapter 17, pp. Unit 17.3. - Publication Year :
- 2001
-
Abstract
- This unit presents protocols designed to detect interacting proteins. Using yeast as a "test tube" and transcriptional activation of a reporter system, interacting proteins can be identified. The system can also be used to test complex formation for proteins for which there exists a reason to expect interaction.
Details
- Language :
- English
- ISSN :
- 1934-2616
- Volume :
- Chapter 17
- Database :
- MEDLINE
- Journal :
- Current protocols in cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 18228339
- Full Text :
- https://doi.org/10.1002/0471143030.cb1703s08