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BRC-1 acts in the inter-sister pathway of meiotic double-strand break repair.
- Source :
-
EMBO reports [EMBO Rep] 2008 Mar; Vol. 9 (3), pp. 287-92. Date of Electronic Publication: 2008 Jan 25. - Publication Year :
- 2008
-
Abstract
- The breast and ovarian cancer susceptibility protein BRCA1 is evolutionarily conserved and functions in DNA double-strand break (DSB) repair through homologous recombination, but its role in meiosis is poorly understood. By using genetic analysis, we investigated the role of the Caenorhabditis elegans BRCA1 orthologue (brc-1) during meiotic prophase. The null mutant in the brc-1 gene is viable, fertile and shows the wild-type complement of six bivalents in most diakinetic nuclei, which is indicative of successful crossover recombination. However, brc-1 mutants show an abnormal increase in apoptosis and RAD-51 foci at pachytene that are abolished by loss of spo-11 function, suggesting a defect in meiosis rather than during premeiotic DNA replication. In genetic backgrounds in which chiasma formation is abrogated, such as him-14/MSH4 and syp-2, loss of brc-1 leads to chromosome fragmentation suggesting that brc-1 is dispensable for crossing over but essential for DSB repair through inter-sister recombination.
- Subjects :
- Animals
Apoptosis
Caenorhabditis elegans enzymology
Embryo Loss
Endodeoxyribonucleases
Esterases metabolism
Indoles
Meiotic Prophase I
Mutation genetics
Rad51 Recombinase metabolism
Caenorhabditis elegans cytology
Caenorhabditis elegans Proteins metabolism
Crossing Over, Genetic
DNA Breaks, Double-Stranded
DNA Repair
Meiosis
Subjects
Details
- Language :
- English
- ISSN :
- 1469-221X
- Volume :
- 9
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- EMBO reports
- Publication Type :
- Academic Journal
- Accession number :
- 18219312
- Full Text :
- https://doi.org/10.1038/sj.embor.7401167