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Development of drug resistance in the population of colon cancer cells under the effect of multifunctional protein YB-1.
- Source :
-
Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2007 Apr; Vol. 143 (4), pp. 463-6. - Publication Year :
- 2007
-
Abstract
- The effects of YB-1 gene on the expression level of P-glycoprotein and drug resistance of tumor cells were studied in cultured HCT116 colon cancer cells. Transitory transfection of chimeric YB-1/GFP gene rendered HCT116 cells a selective advantage in a medium with vinblastine, which caused translocation of the chimeric protein into cell nuclei. This was paralleled by an increase in the expression of P-glycoprotein (multiple drug resistance protein).
- Subjects :
- ATP Binding Cassette Transporter, Subfamily B, Member 1 genetics
ATP Binding Cassette Transporter, Subfamily B, Member 1 metabolism
Cell Line, Tumor
Cell Nucleus metabolism
Colonic Neoplasms genetics
Colonic Neoplasms metabolism
Colonic Neoplasms pathology
Drug Resistance, Multiple drug effects
Gene Expression Regulation, Neoplastic drug effects
Green Fluorescent Proteins genetics
Green Fluorescent Proteins metabolism
Humans
Microscopy, Fluorescence
Protein Transport drug effects
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins metabolism
Vinblastine pharmacology
Y-Box-Binding Protein 1 genetics
Y-Box-Binding Protein 1 metabolism
Drug Resistance, Multiple genetics
Drug Resistance, Neoplasm genetics
Y-Box-Binding Protein 1 physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0007-4888
- Volume :
- 143
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Bulletin of experimental biology and medicine
- Publication Type :
- Academic Journal
- Accession number :
- 18214301
- Full Text :
- https://doi.org/10.1007/s10517-007-0157-0