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The role of hemocytes, serine protease inhibitors and pathogen-associated patterns in prophenoloxidase activation in the desert locust, Schistocerca gregaria.
- Source :
-
Peptides [Peptides] 2008 Feb; Vol. 29 (2), pp. 235-41. Date of Electronic Publication: 2007 Dec 07. - Publication Year :
- 2008
-
Abstract
- The prophenoloxidase-activating system is an important component of the innate immune response of insects, involved in wound healing and melanotic encapsulation. In this paper we show that in the desert locust, Schistocerca gregaria, hemocytes, challenged with microbial elicitors, are indispensable for the limited proteolytic activation of prophenoloxidase (proPO) in plasma. In addition, we assessed the influence of serine protease inhibitors on the induction of PO-activity in plasma. While soybean Bowman-Birk inhibitor (SBBI) inhibited the PO activation by laminarin-treated hemocytes, the endogenous pacifastin-related inhibitors, SGPI-1 (S. gregaria pacifastin-related inhibitor-1) and SGPI-2 did not affect the PO-activity under similar conditions. On the other hand, real-time PCR analysis revealed that the transcripts, encoding SGPI-1-3, were more abundant in the fat body of immune challenged animals, as compared to control animals.
- Subjects :
- Animals
Catechol Oxidase blood
Chymotrypsin pharmacology
Dose-Response Relationship, Drug
Enzyme Activation drug effects
Enzyme Precursors blood
Fat Body drug effects
Fat Body metabolism
Female
Gene Expression drug effects
Glucans
Grasshoppers microbiology
Hemocytes chemistry
Hemocytes drug effects
Hemolymph metabolism
Host-Pathogen Interactions
Humans
Insect Proteins genetics
Insect Proteins metabolism
Insect Proteins pharmacology
Lipopolysaccharides pharmacology
Models, Biological
Peptides genetics
Peptides metabolism
Peptides pharmacology
Polysaccharides pharmacology
Serine Proteinase Inhibitors genetics
Serine Proteinase Inhibitors metabolism
Trypsin Inhibitor, Bowman-Birk Soybean pharmacology
Trypsin Inhibitors pharmacology
Catechol Oxidase metabolism
Enzyme Precursors metabolism
Grasshoppers enzymology
Hemocytes metabolism
Serine Proteinase Inhibitors pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0196-9781
- Volume :
- 29
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Peptides
- Publication Type :
- Academic Journal
- Accession number :
- 18207608
- Full Text :
- https://doi.org/10.1016/j.peptides.2007.07.032