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Predominance of Tetragenococcus halophilus as the cause of sugar thick juice degradation.
- Source :
-
Food microbiology [Food Microbiol] 2008 Apr; Vol. 25 (2), pp. 413-21. Date of Electronic Publication: 2007 Nov 13. - Publication Year :
- 2008
-
Abstract
- The industrial storage of sugar thick juice was simulated on a laboratory scale. Terminal Restriction Fragment Length Polymorphism (T-RFLP) analysis and the application of Clone Libraries in parallel with classical microbiology were used to study the bacterial diversity and all revealed a dominance (>99%) of Tetragenococcus halophilus during storage. The degradation of thick juice correlated with the appearance of L-lactic acid and high concentrations of T. halophilus. In addition, pure cultures of T. halophilus induced degradation of sterile thick juice. A specific PCR was developed to detect T. halophilus and industrial thick juice samples from Belgium, Germany and France all contained T. halophilus, suggesting a consistent association of this organism with thick juice. T. halophilus has been known only as a halophile thus far, and this report is the first to show an association of this organism with a sugar-rich environment.
- Subjects :
- Colony Count, Microbial
DNA, Bacterial chemistry
DNA, Bacterial genetics
Food Microbiology
Lactobacillus classification
Lactobacillus genetics
Phylogeny
RNA, Ribosomal, 16S genetics
Species Specificity
Carbohydrate Metabolism
Food Preservation methods
Industrial Microbiology methods
Lactobacillus metabolism
Polymorphism, Restriction Fragment Length
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9998
- Volume :
- 25
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Food microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 18206785
- Full Text :
- https://doi.org/10.1016/j.fm.2007.10.012