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Expression of coronin-3 (coronin-1C) in diffuse gliomas is related to malignancy.
- Source :
-
The Journal of pathology [J Pathol] 2008 Mar; Vol. 214 (4), pp. 415-24. - Publication Year :
- 2008
-
Abstract
- Coronin-3 (coronin-1C), a homotrimeric F-actin binding protein, has been shown to be important for cell migration and brain morphogenesis. Here, we present for the first time a detailed analysis of the expression pattern of coronin-3 in human brain tumours and demonstrate that coronin-3 expression correlates with malignant phenotype in diffuse gliomas. In general, the expression of coronin-3 varies in different brain tumour entities. However, in diffuse gliomas, the number of coronin-3 expressing tumour cells correlates with the degree of malignancy. High-grade gliomas, such as anaplastic astrocytomas, anaplastic oligodendrogliomas, anaplastic oligoastrocytomas and glioblastomas, show high numbers of tumour cells positive for coronin-3, while diffuse low-grade gliomas, such as diffuse astrocytomas, oligodendrogliomas and oligoastrocytomas, exhibit low numbers of coronin-3-positive tumour cells. In order to explore and verify a contribution of coronin-3 to the malignant phenotype of diffuse gliomas, we employed an efficient shRNA-mediated coronin-3 knockdown in U373 and A172 human glioblastoma cells. Coronin-3 knockdown glioblastoma cells exhibited reduced levels of cell proliferation, cell motility and invasion into extracellular matrix compared to control cells. Together, our findings demonstrate evidence for a contribution of coronin-3 expression in the malignant progression of diffuse gliomas.
- Subjects :
- Astrocytes metabolism
Biomarkers, Tumor deficiency
Biomarkers, Tumor genetics
Biomarkers, Tumor physiology
Brain Neoplasms pathology
Cell Movement
Cell Proliferation
Extracellular Matrix pathology
Glioma pathology
Humans
Matrix Metalloproteinases metabolism
Microfilament Proteins deficiency
Microfilament Proteins genetics
Microfilament Proteins physiology
Neoplasm Invasiveness
Neoplasm Proteins deficiency
Neoplasm Proteins genetics
Neoplasm Proteins metabolism
Neoplasm Proteins physiology
Tumor Cells, Cultured
Biomarkers, Tumor metabolism
Brain Neoplasms metabolism
Glioma metabolism
Microfilament Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0022-3417
- Volume :
- 214
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The Journal of pathology
- Publication Type :
- Academic Journal
- Accession number :
- 18189330
- Full Text :
- https://doi.org/10.1002/path.2308