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The sequential activation of the yeast HOG and SLT2 pathways is required for cell survival to cell wall stress.
- Source :
-
Molecular biology of the cell [Mol Biol Cell] 2008 Mar; Vol. 19 (3), pp. 1113-24. Date of Electronic Publication: 2008 Jan 09. - Publication Year :
- 2008
-
Abstract
- Yeast mitogen-activated protein kinase (MAPK) signaling pathways transduce external stimuli into cellular responses very precisely. The MAPKs Slt2/Mpk1 and Hog1 regulate transcriptional responses of adaptation to cell wall and osmotic stresses, respectively. Unexpectedly, we observe that the activation of a cell wall integrity (CWI) response to the cell wall damage caused by zymolyase (beta-1,3 glucanase) requires both the HOG and SLT2 pathways. Zymolyase activates both MAPKs and Slt2 activation depends on the Sho1 branch of the HOG pathway under these conditions. Moreover, adaptation to zymolyase requires essential components of the CWI pathway, namely the redundant MAPKKs Mkk1/Mkk2, the MAPKKK Bck1, and Pkc1, but it does not require upstream elements, including the sensors and the guanine nucleotide exchange factors of this pathway. In addition, the transcriptional activation of genes involved in adaptation to cell wall stress, like CRH1, depends on the transcriptional factor Rlm1 regulated by Slt2, but not on the transcription factors regulated by Hog1. Consistent with these findings, both MAPK pathways are essential for cell survival under these circumstances because mutant strains deficient in different components of both pathways are hypersensitive to zymolyase. Thus, a sequential activation of two MAPK pathways is required for cellular adaptation to cell wall damage.
- Subjects :
- Adaptation, Physiological drug effects
Cell Wall drug effects
Enzyme Activation drug effects
Hydrolases pharmacology
MAP Kinase Signaling System drug effects
Mutation genetics
Phosphorylation drug effects
Saccharomyces cerevisiae drug effects
Saccharomyces cerevisiae Proteins genetics
Transcriptional Activation drug effects
Cell Wall enzymology
Microbial Viability drug effects
Mitogen-Activated Protein Kinases metabolism
Saccharomyces cerevisiae cytology
Saccharomyces cerevisiae enzymology
Saccharomyces cerevisiae Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1939-4586
- Volume :
- 19
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Molecular biology of the cell
- Publication Type :
- Academic Journal
- Accession number :
- 18184748
- Full Text :
- https://doi.org/10.1091/mbc.E07-08-0742