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Overexpression of SOX15 inhibits proliferation of NT2/D1 cells derived from a testicular embryonal cell carcinoma.
- Source :
-
Molecules and cells [Mol Cells] 2007 Dec 31; Vol. 24 (3), pp. 323-8. - Publication Year :
- 2007
-
Abstract
- SOX (Sry-related HMG box) family proteins, which have an evolutionarily conserved DNA binding domain, have crucial roles in cell differentiation. However, their target genes remain enigmatic. Some members of the SOX family may have roles in regulation of cell proliferation. We established stable NT2/D1 cell lines overexpressing SOX15 (SOX15-NT2/D1), and a modified 3-(4,5-dime-thylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that the SOX15-NT2/D1 cells exhibited significantly slower growth than the controls. Flow cytometry analysis revealed that an increased fraction of the SOX15-NT2/D1 cells were in G1-G0. In addition, a microarray analysis identified 26 genes that were up-regulated in the SOX15-NT2/D1 cells, but none that were down-regulated genes. Among the up-regulated genes, IGFBP5, S100A4, ID2, FABP5, MTSS1, PDCD4 have been shown to be related to cell proliferation and/or the cell cycle.
- Subjects :
- Carcinoma, Embryonal pathology
Cell Line, Tumor
Flow Cytometry
Gene Expression
Humans
Male
Oligonucleotide Array Sequence Analysis
RNA, Messenger metabolism
Reverse Transcriptase Polymerase Chain Reaction
SOX Transcription Factors
Testicular Neoplasms pathology
Cell Proliferation drug effects
DNA-Binding Proteins biosynthesis
High Mobility Group Proteins biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1016-8478
- Volume :
- 24
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Molecules and cells
- Publication Type :
- Academic Journal
- Accession number :
- 18182846