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Sequence variation occurs in CD4 epitopes during early HIV infection.
- Source :
-
Journal of acquired immune deficiency syndromes (1999) [J Acquir Immune Defic Syndr] 2007 Nov 01; Vol. 46 (3), pp. 261-7. - Publication Year :
- 2007
-
Abstract
- Objective: To determine whether viral sequence variation occurs in HIV-specific CD4 epitopes during early HIV infection.<br />Methods: Gag, Nef, and integrase (Int) sequences were obtained from the plasma of 7 subjects identified during acute HIV-1 infection. Changes in the viral sequence were determined based on comparison of sequences obtained at 2 time points during early infection. Peptide-specific CD4+ T-cell responses were measured at matched time points using interferon-gamma enzyme-linked immunosorbent spot assays to identify CD4 epitopes.<br />Results: An average of 4 mutations were identified per subject. The majority of the mutations were nonsynonymous and resulted in a total of 6 amino acid changes in Gag, 7 changes in Nef, and 6 changes and a deletion in Int. Half of the sequence changes were within recognized CD4 epitopes. Mutations within CD4 epitopes were coincident with changes in the peptide-specific CD4 response.<br />Conclusion: These data indicate that sequence variation occurs within recognized CD4 epitopes during early HIV infection. Furthermore, it suggests that mutations within HIV-specific CD4 epitopes may affect T helper cell function.
Details
- Language :
- English
- ISSN :
- 1525-4135
- Volume :
- 46
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of acquired immune deficiency syndromes (1999)
- Publication Type :
- Academic Journal
- Accession number :
- 18167642
- Full Text :
- https://doi.org/10.1097/qai.0b013e3181514427