Short-Term culture of in vitro produced bovine preimplantation embryos with insulin-like growth factor-i prevents heat shock-induced apoptosis through activation of the Phosphatidylinositol 3-Kinase/Akt pathway.

Insulin-like growth factor (IGF-I) has been implicated as a thermoprotective molecule for the preimplantation bovine embryo. Here, it was shown that effects of heat shock (41 degrees C for 15 hr) on induction of apoptosis and reduction in cell number in bovine embryos collected at Day 5 after fertilization were blocked by addition of 100 ng/ml IGF-I at the initiation of heat shock. This action of IGF-I to block heat shock-induced apoptosis was eliminated if embryos were cultured with either a phosphatidylinositol 3-kinase (PI3K) inhibitor (wortmannin) or an Akt inhibitor (1L-6-hydroxymethyl-chiro-inositol 2-(R)-2-o-methyl-3-o-octadecylcarbonate). Immunofluorescence microscopy confirmed the expression of phosphorylated Akt for IGF-I and control embryos. Immunoblotting using an antibody to Akt (phospho S473) indicated increased phosphorylation of Akt in IGF-I-treated embryos. In conclusion, short-term treatment of embryos with IGF-I can block induction of apoptosis caused by heat shock through signaling events requiring PI3K and Akt.
((c) 2007 Wiley-Liss, Inc.)