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Autofluorescent proteins.

Authors :
Dobbie IM
Lowndes NF
Sullivan KF
Source :
Methods in cell biology [Methods Cell Biol] 2008; Vol. 85, pp. 1-22.
Publication Year :
2008

Abstract

Autofluorescent proteins (AFPs) have revolutionized molecular cell biology, and applications continue to harness the power of these genetically encoded fluorescent tags. Here, we review the discovery and physical properties of AFPs as well as their development through mutational optimization for several functional parameters. A practical guide to selection and use of major AFPs is provided as well as an overview of techniques for experimental applications.

Subjects

Subjects :
Amino Acid Sequence
Fluorescent Dyes
Molecular Sequence Data
Protein Conformation
Proteins chemistry
Recombinant Fusion Proteins chemistry
Recombinant Fusion Proteins metabolism
Sequence Alignment
Fluorescence Recovery After Photobleaching methods
Fluorescence Resonance Energy Transfer methods
Green Fluorescent Proteins chemistry
Green Fluorescent Proteins metabolism
Luminescent Proteins chemistry
Luminescent Proteins metabolism
Proteins metabolism

Details

Language :
English
ISSN :
0091-679X
Volume :
85
Database :
MEDLINE
Journal :
Methods in cell biology
Publication Type :
Academic Journal
Accession number :
18155456
Full Text :
https://doi.org/10.1016/S0091-679X(08)85001-7
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