Back to Search
Start Over
Leucine-rich amelogenin peptide induces osteogenesis in mouse embryonic stem cells.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2008 Feb 29; Vol. 367 (1), pp. 1-6. Date of Electronic Publication: 2007 Dec 18. - Publication Year :
- 2008
-
Abstract
- Leucine-rich amelogenin peptide (LRAP), an alternatively spliced amelogenin protein, possesses a signaling property shown to induce osteogenic differentiation. In the current study, we detected LRAP expression during osteogenesis of wild-type (WT) embryonic stem (ES) cells and observed the absence of LRAP expression in amelogenin-null (KO) ES cells. We explored the signaling effect of LRAP on wild-type ES cells, and the ability of LRAP to rescue the impaired osteogenesis phenotype observed in KO ES cells. Our data indicate that LRAP treatment of WT and KO ES cells induces a significant increase in mineral matrix formation, and significant increases in bone sialoprotein and osterix gene expression. In addition, the amelogenin KO phenotype is partially rescued by the addition of exogenous LRAP. These data suggest a unique function of LRAP during ES cell differentiation along osteogenic lineage.
- Subjects :
- Animals
Base Sequence
Bone Regeneration physiology
Cell Differentiation physiology
Embryonic Stem Cells cytology
Gene Expression Regulation, Developmental physiology
Mice
Osteoblasts cytology
Osteoblasts metabolism
Osteocalcin metabolism
Osteogenesis physiology
Phenotype
Sialoglycoproteins biosynthesis
Time Factors
Bone Regeneration drug effects
Cell Differentiation drug effects
Cell Proliferation drug effects
Dental Enamel Proteins pharmacology
Embryonic Stem Cells metabolism
Gene Expression Regulation, Developmental drug effects
Osteogenesis drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 367
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 18086559
- Full Text :
- https://doi.org/10.1016/j.bbrc.2007.12.048