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Evaluation of the NucliSens miniMAG RNA extraction and real-time NASBA applications for the detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae in throat swabs.
- Source :
-
Journal of microbiological methods [J Microbiol Methods] 2008 Feb; Vol. 72 (2), pp. 217-9. Date of Electronic Publication: 2007 Nov 21. - Publication Year :
- 2008
-
Abstract
- Nucleic acids were extracted from 215 throat swabs from patients with community-acquired pneumonia by the manual Boom extraction, the NucliSens miniMAG and the Qiagen DNA blood kit and amplified respectively by in-house real-time NASBA, NucliSens EasyQ reagents, and real-time PCR for the detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae. Five out of 215 throat swabs were found to be C. pneumoniae positive by all techniques used. A total of 11 out of 215 throat swabs were positive for M. pneumoniae; 10/215 by Qiagen extraction and PCR amplification and 9/215 by NucliSens miniMAG and NucliSens EasyQ amplification. The NucliSens miniMAG and NucliSens EasyQ applications were successfully coupled to detect both organisms in throat swabs.
- Subjects :
- Chlamydophila pneumoniae genetics
Humans
Mycoplasma pneumoniae genetics
Polymerase Chain Reaction methods
RNA, Bacterial genetics
Chlamydophila pneumoniae isolation & purification
Mycoplasma pneumoniae isolation & purification
Pharynx microbiology
RNA, Bacterial isolation & purification
Reagent Kits, Diagnostic
Self-Sustained Sequence Replication methods
Subjects
Details
- Language :
- English
- ISSN :
- 0167-7012
- Volume :
- 72
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of microbiological methods
- Publication Type :
- Academic Journal
- Accession number :
- 18077024
- Full Text :
- https://doi.org/10.1016/j.mimet.2007.11.008