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Aging alters gene expression of growth and remodeling factors in human skeletal muscle both at rest and in response to acute resistance exercise.
- Source :
-
Physiological genomics [Physiol Genomics] 2008 Feb 19; Vol. 32 (3), pp. 393-400. Date of Electronic Publication: 2007 Dec 11. - Publication Year :
- 2008
-
Abstract
- The purpose of this investigation was to compare expression of genes that function in inflammation and stress, cell structure and signaling, or remodeling and growth in skeletal muscle of young (32 +/- 7 yr, n = 15) and elderly (72 +/- 5 yr, n = 16) healthy subjects before and after a bout of resistance leg exercises. A real-time RT-PCR method was used to screen 100 transcripts in v. lateralis biopsies obtained before and 72 h postexercise. The screen identified 15 candidates for differential expression due to aging and/or exercise that were measured quantitatively. The median levels of four mRNAs (insulin-like growth factor-1 and its binding protein IGFBP5, ciliary neurotrophic factor, and the metallopeptidase MMP2) were significantly affected by aging and were greater (1.6- to 2.3-fold, P </= 0.05) in the young than elderly muscle at both time points. The median levels of three mRNAs were significantly (P </= 0.05) affected by exercise in the young. The metallopeptidase inhibitor TIMP1 and alpha-cardiac actin mRNAs increased 2-fold and 6.5-fold, respectively, and GDF8 (myostatin) mRNA decreased by 50%. However, elderly muscle did not display any significant changes in gene expression postexercise. Thus, aging muscle shows decreased levels at rest and an impaired response to exercise for a number of mRNAs for factors potentially involved in muscle growth and remodeling. Future studies must determine the functional importance of these gene expression changes to protein synthesis, satellite cell activity, and other processes that are directly involved in the mechanisms of muscle hypertrophy.
- Subjects :
- Actins biosynthesis
Actins genetics
Adult
Aged
Aging metabolism
Ciliary Neurotrophic Factor biosynthesis
Ciliary Neurotrophic Factor genetics
Gene Expression Profiling
Humans
Insulin-Like Growth Factor Binding Protein 5 biosynthesis
Insulin-Like Growth Factor Binding Protein 5 genetics
Insulin-Like Growth Factor I biosynthesis
Insulin-Like Growth Factor I genetics
Intercellular Signaling Peptides and Proteins biosynthesis
Male
Matrix Metalloproteinase 2 biosynthesis
Matrix Metalloproteinase 2 genetics
Muscle Proteins biosynthesis
Muscle, Skeletal metabolism
Myostatin
RNA, Messenger biosynthesis
RNA, Messenger genetics
Reverse Transcriptase Polymerase Chain Reaction
Tissue Inhibitor of Metalloproteinase-1 biosynthesis
Tissue Inhibitor of Metalloproteinase-1 genetics
Transforming Growth Factor beta biosynthesis
Transforming Growth Factor beta genetics
Aging genetics
Gene Expression Regulation physiology
Intercellular Signaling Peptides and Proteins genetics
Muscle Proteins genetics
Muscle, Skeletal growth & development
Rest physiology
Weight Lifting physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1531-2267
- Volume :
- 32
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Physiological genomics
- Publication Type :
- Academic Journal
- Accession number :
- 18073271
- Full Text :
- https://doi.org/10.1152/physiolgenomics.00191.2007