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Isolation and characterization of strain of Bacillus thuringiensis subsp. kenyae containing two novel cry1-type toxin genes.
- Source :
-
Journal of microbiology and biotechnology [J Microbiol Biotechnol] 2007 Sep; Vol. 17 (9), pp. 1498-503. - Publication Year :
- 2007
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Abstract
- To identify novel crystal proteins, Bacillus thuringiensis 2385-1 was isolated from Korean soil samples and characterized. The H-serotype of 2385-1 was identical to that of subsp. kenyae (H4a4c), and its crystal toxin was bipyramidal-shaped. However, 2385-1 showed a much higher toxicity towards Plutella xylostella and Spodoptera exigua larvae than subsp. kenyae. In addition, the crystal protein profile and plasmid DNA pattern of 2385-1 differed from those of subsp. kenyae. To verify the crystal protein gene types of 2385-1, a PCR-RFLP analysis was performed, and the results revealed that 2385-1 contained two novel cry1-type crystal protein genes, cry1-5 and cry1-12, in addition to the cry1Ja1 gene. The deduced amino acid sequences of cry1-5 and cry1-12 showed a 97.9% and 75.7% sequence similarity with the CrylAb and Cry1Ja crystal proteins, respectively. Among the novel crystal proteins, Cry1-5 showed a high toxicity towards P. xylostella and S. exigua larvae. In conclusion, B. thuringiensis 2385-1 is a new isolate in terms of its gene types, and should be a promising source for an insecticide to control lepidopteran larvae.
- Subjects :
- Animals
Bacillus thuringiensis Toxins
Endotoxins pharmacology
Endotoxins toxicity
Genes, Bacterial
Insecticides toxicity
Lepidoptera drug effects
Lepidoptera microbiology
Spodoptera drug effects
Spodoptera microbiology
Bacillus thuringiensis chemistry
Bacillus thuringiensis genetics
Bacterial Proteins genetics
Bacterial Proteins toxicity
Bacterial Toxins genetics
Bacterial Toxins toxicity
Endotoxins genetics
Hemolysin Proteins genetics
Hemolysin Proteins toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 1017-7825
- Volume :
- 17
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Journal of microbiology and biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 18062228