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Lymph node and pulmonary metastases after transplantation of oral squamous cell carcinoma cell line (HSC-3) into the subcutaneous tissue of nude mouse: detection of metastases by genetic methods using beta-globin and mutant p53 genes.

Authors :
Shigeta T
Umeda M
Komatsubara H
Komori T
Source :
Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics [Oral Surg Oral Med Oral Pathol Oral Radiol Endod] 2008 Apr; Vol. 105 (4), pp. 486-90. Date of Electronic Publication: 2007 Dec 03.
Publication Year :
2008

Abstract

We attempted to establish an in vivo experimental model of metastasis of oral caner. The HSC-3 cell line, originating from human oral squamous cell carcinoma, was transplanted into nude mice, and metastases to the lymph node and the lung were investigated using genetic analysis. The HSC-3 cells, transplanted subcutaneously into the lateral back of nude mice, and punctured repeatedly after cyst formation, could metastasize to the lymph node and the lung. Genetic analysis using human beta-globin gene demonstrated that the frequency of metastasis to the lymph node and the lung at 12 weeks after the transplantation was 45% and 40%, respectively. Mutant allele-specific amplification of p53 gene could also detect micrometastasis with almost equal sensitivity of polymerase chain reaction with beta-globin gene. Subcutaneous transplantation is easy with excellent reproducibility compared with intravenous or orthotopic transplantation, and we think that this experimental model can be one of standard models of cancer metastasis.

Details

Language :
English
ISSN :
1528-395X
Volume :
105
Issue :
4
Database :
MEDLINE
Journal :
Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics
Publication Type :
Academic Journal
Accession number :
18061490
Full Text :
https://doi.org/10.1016/j.tripleo.2007.06.016