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Cloning of fibrinolytic enzyme gene from Bacillus subtilis isolated from Cheonggukjang and its expression in protease-deficient Bacillus subtilis strains.
- Source :
-
Journal of microbiology and biotechnology [J Microbiol Biotechnol] 2007 Jun; Vol. 17 (6), pp. 1018-23. - Publication Year :
- 2007
-
Abstract
- Bacillus subtilis CH3-5 was isolated from cheonggukjang prepared according to traditional methods. CH3-5 secreted at least four different fibrinolytic proteases (63, 47, 29, and 20 kDa) into the culture medium. A fibrinolytic enzyme gene, aprE2, encoding a 29 kDa enzyme was cloned from the genomic DNA of CH3-5, and the DNA sequence determined. aprE2 was overexpressed in heterologous B. subtilis strains deficient in extracellular proteases using a E. coli-Bacillus shuttle vector. A 29 kDa AprE2 band was observed and AprE2 seemed to exhibit higher activities towards fibrin rather than casein.
- Subjects :
- Amino Acid Sequence
Base Sequence
Caseins metabolism
Cloning, Molecular
Electrophoresis, Polyacrylamide Gel
Molecular Sequence Data
Molecular Weight
Recombinant Proteins biosynthesis
Bacillus subtilis genetics
Fermentation
Fibrin metabolism
Peptide Hydrolases genetics
Glycine max microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1017-7825
- Volume :
- 17
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of microbiology and biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 18050921