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FOXP3 mRNA expression analysis in the peripheral blood and allograft of heart transplant patients.
- Source :
-
Transplant immunology [Transpl Immunol] 2008 Jan; Vol. 18 (3), pp. 250-4. Date of Electronic Publication: 2007 Aug 31. - Publication Year :
- 2008
-
Abstract
- Previously, we demonstrated in heart transplant patients that FOXP3, a gene required for the development and function of regulatory T cells, was highly expressed in the graft during an acute cellular rejection. In this study, we analyzed whether the FOXP3 gene expression in the peripheral blood also reflects anti-donor immune responses, and therefore may provide clues for non-invasive detection of non-responsiveness or acute rejection. We examined the FOXP3 expression patterns of peripheral blood mononuclear cells (PBMC; n=69) of 19 heart transplant patients during quiescence and rejection in comparison with those of endomyocardial biopsies (EMB; n=75) of 24 heart transplant patients. While the FOXP3 mRNA levels were abundantly expressed in rejecting EMB (ISHLT rejection grade>1R) compared with EMB without histological evidence of myocardial damage (ISHLT rejection grade 0R-1R; p=0.003), no association with rejection or non-responsiveness was found for the FOXP3 mRNA levels in the peripheral blood. Thus, in contrast to intragraft FOXP3 gene expression, the peripheral FOXP3 mRNA levels lack correlation with anti-donor immune responses in the graft, and, consequently, FOXP3 does not appear to be a potential candidate gene for non-invasive diagnosis of non-responsiveness or rejection.
- Subjects :
- Adult
Aged
Biopsy
Female
Forkhead Transcription Factors blood
Gene Expression
Graft Survival immunology
Humans
Male
Middle Aged
Myocardium pathology
RNA, Messenger blood
RNA, Messenger genetics
Forkhead Transcription Factors genetics
Graft Rejection
Heart Transplantation immunology
Leukocytes, Mononuclear metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0966-3274
- Volume :
- 18
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Transplant immunology
- Publication Type :
- Academic Journal
- Accession number :
- 18047933
- Full Text :
- https://doi.org/10.1016/j.trim.2007.08.001