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Real-time PCR for type-specific identification of herpes simplex in clinical samples: evaluation of type-specific results in the context of CNS diseases.
- Source :
-
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology [J Clin Virol] 2008 Feb; Vol. 41 (2), pp. 87-91. Date of Electronic Publication: 2007 Nov 26. - Publication Year :
- 2008
-
Abstract
- Background: HSV-1 and HSV-2 cause CNS infections of dissimilar clinico-pathological characteristics with prognostic and therapeutic implications.<br />Objectives: To validate a type-specific real-time PCR that uses MGB/LNA Taqman probes and to review the virologico-clinical data of 25 eligible patients with non-neonatal CNS infections.<br />Results: This real-time PCR was evaluated against conventional PCR (26 CSF and 20 quality controls), and LightCycler assay (51 mucocutaneous, 8 CSF and 32 quality controls) and culture/immunofluorescence (75 mucocutaneous) to assess typing with independent methods. Taqman real-time PCR detected 240 HSV genomes per ml CSF, a level appropriate for the management of patients, and provided unambiguous typing for the 104 positive (62 HSV-1 and 42 HSV-2) out the 160 independent clinical samples tested. HSV type diagnosed by Taqman real-time PCR predicted final diagnosis (meningitis versus encephalitis/meningoencephalitis, p<0.001) in 24/25 patients at time of presentation, in contrast to clinical evaluation.<br />Conclusions: Our real-time PCR, as a sensitive and specific means for type-specific HSV diagnosis, provided rapid prognostic information for patient management.
- Subjects :
- Base Sequence
DNA Probes
Encephalitis, Viral virology
Herpes Simplex virology
Herpesvirus 1, Human genetics
Herpesvirus 1, Human isolation & purification
Herpesvirus 2, Human genetics
Herpesvirus 2, Human isolation & purification
Humans
Meningitis, Viral virology
Molecular Sequence Data
Species Specificity
Viral Envelope Proteins chemistry
Viral Envelope Proteins genetics
Encephalitis, Viral diagnosis
Herpes Simplex diagnosis
Herpesvirus 1, Human classification
Herpesvirus 2, Human classification
Meningitis, Viral diagnosis
Polymerase Chain Reaction methods
Subjects
Details
- Language :
- English
- ISSN :
- 1386-6532
- Volume :
- 41
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
- Publication Type :
- Academic Journal
- Accession number :
- 18037340
- Full Text :
- https://doi.org/10.1016/j.jcv.2007.10.010