Back to Search
Start Over
[Influence of transforming growth factor-beta1 inducing time on chondrogenesis of bone marrow stromal cells (BMSCs): in vitro experiment with porcine BMSCs].
- Source :
-
Zhonghua yi xue za zhi [Zhonghua Yi Xue Za Zhi] 2007 Aug 21; Vol. 87 (31), pp. 2218-22. - Publication Year :
- 2007
-
Abstract
- Objective: To explore the influence of transforming growth factor (TGF)-beta1 inducing time on the chondrogenesis of bone marrow stromal cells (BMSC), and on the construction of tissue engineering cartilage.<br />Methods: BMSCs were obtained from the greater trochanters of 3 pigs, cultured, seeded onto the cylindrical scaffolds made of polyglycolic acid at the density of 5.0 x 10(7) cells/ml, and then cultured with chondrogenesis media containing TGF-beta(1) (10 ng/ml), insulin-like growth factor-I (50 microg/L), and dexamethasone (40 microg/L) to be induced by TGF-beta(1) for 2 weeks (Group A), 4 weeks (Group B), 6 weeks (Group C), 8 weeks (Group D), or 10 weeks (Group E) respectively. 10 weeks later the cylindrical scaffolds underwent gross observation and histological examination. Alcin blue method was used to examine the content of proteoglycan (GAG). Immunochemistry and Western blotting were used to examine the type II collagen. The cylindrical scaffolds underwent biomechanical analysis.<br />Results: HE staining showed cartilage lacunae increasing in number from the periphery to center of the cylindrical scaffolds with the extended inducing time, and were arranged more uniformly progressively. Histochemical staining showed GAG accumulation. Immunohistochemistry and Western blotting showed that the content of type II collagen increased gradually, the amount of type II collagen stabilized after 6 weeks' culture, and there was no significant difference in the content of type II collagen between Group C and Group E. Biomechanical analysis showed that the modulus of elasticity and compression strength of the groups induced for more than 6 weeks (Groups C, D, and E) were all higher then those of Groups and B.<br />Conclusion: TGF-beta(1) inducing time is correlated with the cartilage engineering characteristics with BMSC as seed cells. Induction for 6 weeks helps construct tissue engineered cartilage with good tissue structure, chemical composition and biomechanical properties.
- Subjects :
- Animals
Blotting, Western
Bone Marrow Cells cytology
Bone Marrow Cells metabolism
Cells, Cultured
Chondrocytes cytology
Chondrocytes drug effects
Chondrocytes metabolism
Collagen Type II metabolism
Immunohistochemistry
Proteoglycans metabolism
Stromal Cells cytology
Stromal Cells metabolism
Swine
Time Factors
Tissue Engineering methods
Bone Marrow Cells drug effects
Chondrogenesis drug effects
Stromal Cells drug effects
Transforming Growth Factor beta pharmacology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0376-2491
- Volume :
- 87
- Issue :
- 31
- Database :
- MEDLINE
- Journal :
- Zhonghua yi xue za zhi
- Publication Type :
- Academic Journal
- Accession number :
- 18001534