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Mitochondrial metabolism of 17alpha-hydroxyprogesterone in male sea bass (Dicentrarchus labrax): a potential target for endocrine disruptors.
- Source :
-
Aquatic toxicology (Amsterdam, Netherlands) [Aquat Toxicol] 2007 Dec 30; Vol. 85 (4), pp. 258-66. Date of Electronic Publication: 2007 Oct 30. - Publication Year :
- 2007
-
Abstract
- The metabolism of 17alpha-hydroxyprogesterone (17P(4)) was investigated in different subcellular fractions isolated from male gonads of sea bass (Dicentrarchus labrax L). The existence of CYP17 (C17,20-lyase activity) and CYP11B (11beta-hydroxylase) catalyzed reactions was demonstrated in the mitochondrial fraction, where 17P(4) was converted to androstenedione (AD) and further metabolized to 11beta-hydroxyandrostenedione (betaAD). The synthesis of betaAD predominated in early spermatogenic testis, indicating a role of betaAD in testicular recrudescence. Additionally, the in vitro effect of model endocrine disrupting chemicals (i.e. nonylphenol (NP), p,p'-DDE, benzo[a]anthracene (BaA), tributyltin (TBT) and ketoconazole (KCZ)) on the mitochondrial metabolism of 17P(4) was investigated. Among the tested compounds, 100 microM NP inhibited the activity of CYP17 (C17,20-lyase) whereas 100 microM KCZ inhibited both CYP17 and CYP11B. Both chemicals showed the potential to disrupt the reproductive cycle of fish living in polluted environments due to impairment of testicular steroid biosynthesis. These results suggest that mitochondrial metabolism of 17P(4) may constitute a new sensitive probe for the assessment of endocrine disruption in fish.
- Subjects :
- Animals
Benz(a)Anthracenes pharmacology
Dichlorodiphenyl Dichloroethylene pharmacology
Gas Chromatography-Mass Spectrometry
Histocytochemistry
Male
Mitochondria enzymology
Phenols pharmacology
Steroid 11-beta-Hydroxylase antagonists & inhibitors
Steroid 11-beta-Hydroxylase metabolism
Steroid 17-alpha-Hydroxylase antagonists & inhibitors
Steroid 17-alpha-Hydroxylase metabolism
Testis enzymology
Trialkyltin Compounds pharmacology
17-alpha-Hydroxyprogesterone metabolism
Bass metabolism
Endocrine Disruptors pharmacology
Mitochondria metabolism
Testis metabolism
Water Pollutants, Chemical pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0166-445X
- Volume :
- 85
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Aquatic toxicology (Amsterdam, Netherlands)
- Publication Type :
- Academic Journal
- Accession number :
- 17977610
- Full Text :
- https://doi.org/10.1016/j.aquatox.2007.09.010