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[Study on extraction and antigenicity of the outer-membrane-protein of Bordetella avium].
- Source :
-
Wei sheng wu xue bao = Acta microbiologica Sinica [Wei Sheng Wu Xue Bao] 2007 Aug; Vol. 47 (4), pp. 714-7. - Publication Year :
- 2007
-
Abstract
- To study the immunogenicity of Bordetella avium OMP, ultrasonic dispersion, TritonX-100 technique were used to extract Bordetella avium OMP. Its content was determined by Bradford method and it was detected by SDS-PAGE. Then OMP immunizing antigen was prepared and 1-day chicken was vaccinated by hypodermic inoculation, with the immunizing does of 0.3mL (OMP90microg), 0.5mL (OMP150microg), 0.8mL (OMP240microg), respectively. Results showed that content of Bordetella avium OMP is 300 g/mL, the best immunizing does is 0.5mL each one and Chickens can be protected against Bordetella avium at fetal dose if antibody titer is over 1:2(8). We can see from the antibody level detected by indirect ELISA that antibody can last long enough to help chickens pass susceptible period, so OMP has good immunogenicity. Results of this study lay good foundation for the development of monoclonal antibody to OMP, rapid diagnosis kit and subunit vaccine.
- Subjects :
- Animals
Antibodies, Bacterial blood
Bacterial Outer Membrane Proteins chemistry
Bordetella Infections immunology
Bordetella Infections microbiology
Bordetella Infections prevention & control
Bordetella avium chemistry
Chickens
Immunization
Octoxynol chemistry
Poultry Diseases immunology
Poultry Diseases prevention & control
Rabbits
Bacterial Outer Membrane Proteins immunology
Bacterial Outer Membrane Proteins isolation & purification
Bordetella Infections veterinary
Bordetella avium immunology
Poultry Diseases microbiology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0001-6209
- Volume :
- 47
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Wei sheng wu xue bao = Acta microbiologica Sinica
- Publication Type :
- Academic Journal
- Accession number :
- 17944379