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Impaired hippocampal neurogenesis is involved in cognitive dysfunction induced by thiamine deficiency at early pre-pathological lesion stage.
- Source :
-
Neurobiology of disease [Neurobiol Dis] 2008 Feb; Vol. 29 (2), pp. 176-85. Date of Electronic Publication: 2007 Sep 07. - Publication Year :
- 2008
-
Abstract
- It has not been reported whether thiamine deficiency (TD) affects hippocampal neurogenesis or not. Here, we explored the influence of TD at early pre-pathological lesion stage on hippocampal neurogenesis and the correlation between affected hippocampal neurogenesis and cognitive dysfunction. We prepared TD mouse model by feeding a thiamine-depleted diet. Learning and memory functions of TD mice were tested with Y-maze. Hippocampal neurogenesis was studied with BrdU, PCNA, Dcx, and NeuN immunohistochemical staining. The results showed significant decline in learning ability and hippocampal neurogenesis simultaneously since 9-days of treatment when the model mice did not exhibit regular pathological lesion, the loss of cholinergic neurons, decrease of NeuN-positive hippocampal cell, and abnormal long-term potentiation of hippocampal CA1 and CA3. Re-administering thiamine reversed the weakened learning ability as well as the impaired hippocampal neurogenesis induced by TD at early pre-pathological lesion stage. The present study demonstrated that hippocampal neurogenesis was vulnerable to TD and the impaired hippocampal neurogenesis is greatly involved in cognitive dysfunction induced by TD at early pre-pathological lesion stage.
- Subjects :
- Animals
Behavior, Animal
Bromodeoxyuridine metabolism
Choline O-Acetyltransferase metabolism
Disease Models, Animal
Dose-Response Relationship, Immunologic
Doublecortin Protein
Electric Stimulation
Food, Formulated adverse effects
In Vitro Techniques
Long-Term Potentiation physiology
Long-Term Potentiation radiation effects
Male
Maze Learning physiology
Mice
Mice, Inbred C57BL
Nerve Tissue Proteins metabolism
Patch-Clamp Techniques
Thiamine Deficiency etiology
Time Factors
Cell Proliferation
Cognition Disorders etiology
Cognition Disorders pathology
Hippocampus pathology
Neurons physiology
Thiamine Deficiency complications
Subjects
Details
- Language :
- English
- ISSN :
- 0969-9961
- Volume :
- 29
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Neurobiology of disease
- Publication Type :
- Academic Journal
- Accession number :
- 17936635
- Full Text :
- https://doi.org/10.1016/j.nbd.2007.08.014