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Glial glutamate transporter 1 regulates the spatial and temporal coding of glutamatergic synaptic transmission in spinal lamina II neurons.
- Source :
-
Neuroscience [Neuroscience] 2007 Nov 23; Vol. 149 (4), pp. 898-907. Date of Electronic Publication: 2007 Sep 11. - Publication Year :
- 2007
-
Abstract
- Glutamatergic synaptic transmission is a dynamic process determined by the amount of glutamate released by presynaptic sites, the clearance of glutamate in the synaptic cleft, and the properties of postsynaptic glutamate receptors. Clearance of glutamate in the synaptic cleft depends on passive diffusion and active uptake by glutamate transporters. In this study, we examined the role of glial glutamate transporter 1 (GLT-1) in spinal sensory processing. Excitatory postsynaptic currents (EPSCs) of substantia gelatinosa neurons recorded from spinal slices of young adult rats were analyzed before and after GLT-1 was pharmacologically blocked by dihydrokainic acid. Inhibition of GLT-1 prolonged the EPSC duration and the EPSC decay phase. The EPSC amplitudes were increased in neurons with weak synaptic input but decreased in neurons with strong synaptic input upon inhibition of GLT-1. We suggest that presynaptic inhibition, desensitization of postsynaptic AMPA receptors, and glutamate "spillover" contributed to the kinetic change of EPSCs induced by the blockade of GLT-1. Thus, GLT-1 is a key component in maintaining the spatial and temporal coding in signal transmission at the glutamatergic synapse in substantia gelatinosa neurons.
- Subjects :
- Animals
Benzothiadiazines pharmacology
Dose-Response Relationship, Radiation
Drug Interactions
Electric Stimulation
Enzyme Inhibitors pharmacology
Excitatory Postsynaptic Potentials drug effects
Excitatory Postsynaptic Potentials physiology
Excitatory Postsynaptic Potentials radiation effects
Glutamic Acid pharmacology
In Vitro Techniques
Kainic Acid analogs & derivatives
Kainic Acid pharmacology
Male
Neurons drug effects
Neurons radiation effects
Oligopeptides pharmacology
Patch-Clamp Techniques methods
Rats
Rats, Sprague-Dawley
Synaptic Transmission physiology
Excitatory Amino Acid Transporter 2 physiology
Glutamic Acid metabolism
Neurons physiology
Spinal Cord cytology
Synaptic Transmission drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0306-4522
- Volume :
- 149
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Neuroscience
- Publication Type :
- Academic Journal
- Accession number :
- 17935889
- Full Text :
- https://doi.org/10.1016/j.neuroscience.2007.07.063