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9trans,11trans conjugated linoleic acid inhibits the development of azoxymethane-induced colonic aberrant crypt foci in rats.
- Source :
-
Nutrition and cancer [Nutr Cancer] 2007; Vol. 59 (1), pp. 82-91. - Publication Year :
- 2007
-
Abstract
- We investigated the effects of 9trans,11trans (9t,11t)-conjugated linoleic acid (CLA) isomer on azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) in rats. Male F344 rats were given 2 weekly subcutaneous injections of AOM (20 mg/kg bw) to induce colonic ACF. They also were fed a diet containing either 0.01%, 0.1%, or 1% 9t,11t-CLA for 4 wk starting 1 wk before the first dosing of AOM. The group that received a diet supplemented with 9t,11t-CLA had a significantly lower number of ACF/colon in comparison to the AOM alone group in a dose-dependent manner up to 0.1%. Furthermore, treatment with 9t,11t-CLA induced apoptosis and suppressed cell proliferation activity in the non-lesional crypts. The downregulation of cyclooxygenase-2 and cyclin D1 and the activation of peroxisome proliferators activated receptor gamma were observed in the colonic mucosa of rats fed a diet supplemented with 9t,11t-CLA. Our findings thus provide some novel insight into the chemopreventive effect of 9t,11t-CLA against preinitiation as well as postinitiation stages of colorectal carcinogenesis.
- Subjects :
- Animals
Azoxymethane toxicity
Biomarkers, Tumor analysis
Biomarkers, Tumor metabolism
Cell Division drug effects
Colon chemistry
Colon drug effects
Colonic Neoplasms chemically induced
Colonic Neoplasms epidemiology
Colonic Neoplasms metabolism
Cyclin D1 metabolism
Cyclooxygenase 2 metabolism
Disease Models, Animal
Dose-Response Relationship, Drug
Immunohistochemistry
Intestinal Mucosa metabolism
Lipids analysis
Male
PPAR gamma metabolism
Precancerous Conditions chemically induced
Precancerous Conditions epidemiology
Precancerous Conditions metabolism
Random Allocation
Rats
Rats, Inbred F344
Apoptosis drug effects
Colon pathology
Colonic Neoplasms prevention & control
Linoleic Acids, Conjugated pharmacology
Precancerous Conditions prevention & control
Subjects
Details
- Language :
- English
- ISSN :
- 0163-5581
- Volume :
- 59
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nutrition and cancer
- Publication Type :
- Academic Journal
- Accession number :
- 17927506
- Full Text :
- https://doi.org/10.1080/01635580701419055