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In vitro studies of archaeal translational initiation.
- Source :
-
Methods in enzymology [Methods Enzymol] 2007; Vol. 430, pp. 79-109. - Publication Year :
- 2007
-
Abstract
- Initiation is the step of translation that has incurred the greatest evolutionary divergence. In silico and experimental studies have shown that archaeal translation initiation resembles neither the bacterial nor the eukaryotic paradigm, but shares features with both. The structure of mRNA in archaea is similar to the bacterial one, although the protein factors that assist translational initiation are more numerous than in bacteria and are homologous to eukaryotic proteins. This chapter describes a number of techniques that can be used for in vitro studies of archaeal translation and translational initiation, using as a model system the thermophilic crenarcheon Sulfolobus solfataricus, growing optimally at about 80 degrees in an acidic environment.
- Subjects :
- Archaeal Proteins genetics
Archaeal Proteins metabolism
Macromolecular Substances
Methionine metabolism
Peptide Initiation Factors isolation & purification
Peptide Initiation Factors metabolism
RNA, Archaeal metabolism
RNA, Messenger metabolism
RNA, Transfer isolation & purification
RNA, Transfer metabolism
RNA, Transfer, Met isolation & purification
RNA, Transfer, Met metabolism
Recombinant Proteins genetics
Recombinant Proteins metabolism
Ribosomes chemistry
Ribosomes metabolism
Protein Biosynthesis
Sulfolobus solfataricus genetics
Sulfolobus solfataricus metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0076-6879
- Volume :
- 430
- Database :
- MEDLINE
- Journal :
- Methods in enzymology
- Publication Type :
- Academic Journal
- Accession number :
- 17913636
- Full Text :
- https://doi.org/10.1016/S0076-6879(07)30005-0