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Antifouling activity of sessile bacilli derived from marine surfaces.
- Source :
-
Journal of industrial microbiology & biotechnology [J Ind Microbiol Biotechnol] 2008 Jan; Vol. 35 (1), pp. 9-15. Date of Electronic Publication: 2007 Oct 02. - Publication Year :
- 2008
-
Abstract
- Marine biofilms are a virtually untapped source of bioactive molecules that may find application as novel antifoulants in the marine paint industry. This study aimed at determining the potential of marine biofilm bacteria to produce novel biomolecules with potential application as natural antifoulants. Nine representative strains were isolated from a range of surfaces and were grown in YEB medium and harvested during the late exponential growth phase. Bacterial biomass and spent culture medium were extracted with ethanol and ethyl acetate, respectively. Extracts were assayed for their antifouling activity using two tests: (1) antimicrobial well diffusion test against a common fouling bacterium, Halomonas marina, and (2) anti-crustacean activity test using Artemia salina. Our results showed that none of the ethanolic extracts (bacterial biomass) were active in either test. In contrast, most of the organic extracts had antimicrobial activity (88%) and were toxic towards A. salina (67%). Sequencing of full 16 S ribosomal DNA analysis showed that the isolates were related to Bacillus mojavensis and Bacillus firmus. Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF-MS) profiling of ethyl acetate extracts of culture supernatants showed that these species produce the bioactive lipopeptides surfactin A, mycosubtilin and bacillomycin D.
- Subjects :
- Animals
Anti-Bacterial Agents metabolism
Antimicrobial Cationic Peptides
Bacillus classification
Bacillus genetics
Biofilms growth & development
Calcium Carbonate
Hydrocharitaceae microbiology
Lipopeptides metabolism
Lipoproteins metabolism
Lipoproteins pharmacology
Molecular Sequence Data
Peptides metabolism
Peptides pharmacology
Peptides, Cyclic metabolism
Phylogeny
Plant Leaves microbiology
Sequence Analysis, DNA
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Anti-Bacterial Agents pharmacology
Artemia drug effects
Bacillus isolation & purification
Bacillus metabolism
Biofilms drug effects
Halomonas drug effects
Lipopeptides pharmacology
Peptides, Cyclic pharmacology
Seawater microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1367-5435
- Volume :
- 35
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of industrial microbiology & biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 17909869
- Full Text :
- https://doi.org/10.1007/s10295-007-0260-2