Evaluation of cytomegalovirus (CMV) DNA quantification in dried blood spots: retrospective study of CMV congenital infection.

Authors :: Vauloup-Fellous C
Ducroux A
Couloigner V
Marlin S
Picone O
Galimand J
Loundon N
Denoyelle F
Grangeot-Keros L
Leruez-Ville M
Source :: Journal of clinical microbiology [J Clin Microbiol] 2007 Nov; Vol. 45 (11), pp. 3804-6. Date of Electronic Publication: 2007 Sep 26.
Publication Year :: 2007
Abstract: We compared two protocols for extracting DNA from dried blood spots for cytomegalovirus (CMV) DNA detection and quantification by real-time PCR. Both extraction methods were reliable for the retrospective diagnosis of CMV congenital infection. Quantification of CMV DNA was valuable after normalization of viral loads with albumin gene PCR amplification results.

Subjects :: Child, Preschool
Cytomegalovirus genetics
Humans
Infant
Infant, Newborn
Retrospective Studies
Sensitivity and Specificity
Viral Load
Cytomegalovirus isolation & purification
Cytomegalovirus Infections congenital
Cytomegalovirus Infections diagnosis
DNA, Viral blood
Polymerase Chain Reaction methods

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