Isolation and characterization of a novel cross-infective rhizobia from Sesbania aculeata (Dhaincha).

Authors :: Biswas S
Das RH
Sharma GL
Das HR
Source :: Current microbiology [Curr Microbiol] 2008 Jan; Vol. 56 (1), pp. 48-54. Date of Electronic Publication: 2007 Sep 26.
Publication Year :: 2008
Abstract: The Sesbania has been widely used as green manure to improve the productivity of several crops. Sinorhizobium saheli strain (SB2) was isolated from the root nodule of Sesbania aculeata. The Tn5 mutants (300) of SB2 were generated and studied for their nodulation efficiencies in its specific and cross-infective host plants. The mutant, SB2M3, was found to have two- and four fold higher nodulation efficiency than wild type in parent host and nonspecific host plant, respectively. SB2M3 differed from SB2 in exopolysaccharide and lipopolysaccharide content. SB2M3 was halotolerant and could grow in alkaline pH at comparatively high temperatures. Hence, it may find an application in agritechnology.

Subjects :: DNA Transposable Elements
Hydrogen-Ion Concentration
Lipopolysaccharides analysis
Mutagenesis, Insertional
Plant Roots microbiology
Polysaccharides, Bacterial analysis
Sinorhizobium chemistry
Sinorhizobium genetics
Sinorhizobium physiology
Sodium Chloride metabolism
Sesbania microbiology
Sinorhizobium isolation & purification

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