Back to Search
Start Over
Biochemical and biological properties of DNA photolyases derived from utraviolet-sensitive rice cultivars.
- Source :
-
Genes & genetic systems [Genes Genet Syst] 2007 Aug; Vol. 82 (4), pp. 311-9. - Publication Year :
- 2007
-
Abstract
- Class I and class II CPD photolyases are enzymes which repair pyrimidine dimers using visible light. A detailed characterization of class I CPD photolyases has been carried out, but little is known about the class II enzymes. Photolyases from rice are suitable for functional analyses because systematic breeding for long periods in Asian countries has led to the selection of naturally occurring mutations in the CPD photolyase gene. We report the biochemical characterization of rice mutant CPD photolyases purified as GST-form from Escherichia coli. We identified three amino acid changes, Gln126Arg, Gly255Ser, and Gln296His, among which Gln but not His at 296 is important for complementing phr-defective E. coli, binding UV-damage in E. coli, and binding thymine dimers in vitro. The photolyase with Gln at 296 has an apoenzyme:FAD ratio of 1 : 0.5 and that with His at 296 has an apoenzyme:FAD ratio of 1 : 0.12-0.25, showing a role for Gln at 296 in the binding of FAD not in the binding of thymine dimer. Concerning Gln or Arg at 126, the biochemical activity of the photolyases purified from E. coli and complementing activity for phr-defective E. coli are similarly proficient. However, the sensitivity to UV of cultivars differs depending on whether Gln or Arg is at 126. The role of Gln and Arg at 126 for photoreactivation in rice is discussed.
- Subjects :
- DNA Damage genetics
DNA, Plant genetics
Escherichia coli enzymology
Escherichia coli genetics
Escherichia coli radiation effects
Flavin-Adenine Dinucleotide chemistry
Flavin-Adenine Dinucleotide metabolism
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Mutation genetics
Oryza genetics
Pyrimidine Dimers chemistry
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins metabolism
Sequence Alignment
Sequence Homology, Nucleic Acid
Ultraviolet Rays
Amino Acid Substitution genetics
DNA Repair genetics
Deoxyribodipyrimidine Photo-Lyase genetics
Deoxyribodipyrimidine Photo-Lyase metabolism
Oryza enzymology
Oryza radiation effects
Subjects
Details
- Language :
- English
- ISSN :
- 1341-7568
- Volume :
- 82
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Genes & genetic systems
- Publication Type :
- Academic Journal
- Accession number :
- 17895582
- Full Text :
- https://doi.org/10.1266/ggs.82.311