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[Effects of caveolin-1 on biologic behavior of laryngeal squamous cell carcinoma HEp2 cell line GU].
- Source :
-
Zhonghua zhong liu za zhi [Chinese journal of oncology] [Zhonghua Zhong Liu Za Zhi] 2007 May; Vol. 29 (5), pp. 329-33. - Publication Year :
- 2007
-
Abstract
- Objective: To investigate the effects of caveolin-1 on the biologic behavior of laryngeal squamous cell carcinoma HEp2 cell line in vitro.<br />Methods: Eukaryotic expression vector of human caveolin-1 gene was constructed and transfected into HEp2 cells by Lipofectamine. The clones stably overexpressing caveolin-1 were identified by real-time PCR and Western blotting. Cell proliferation viability was tested by MTT assay. Anchorage-independent growth was determined by assaying colony formation in soft agar. Flow cytometry was used to assess the cell cycle and apoptosis. The relative phosphorylation level of EGFR and ERK1/2 were detected by Western blotting. Localization of caveolin-1 and EGFR were studied by laser confocal laser scanning microscopy.<br />Results: The expression vector of caveolin-1 was constructed and three clones stably overexpressing caveolin-1 were obtained. Comparing with the parental HEp2 cells, the transfected cells exhibited a slower growth rate and formed fewer colonies in soft agar. The results of FACS analysis revealed that overexpression of caveolin-1 resulted in the cell cycle arrest at G0/G1 phase and increased the apoptotic cell fraction. EGFR was found to colocalize with caveolin-1 in transfected cells by confocal laser scanning microscopy and Western blotting results showed that overexpression of caveolin-1 reduced the phosphorylation of EGFR and Erkl/2.<br />Conclusion: Overexpression of caveolin-1 suppresses the growth of HEp2 cells and induces apoptosis and inhibition of EGFR-MAPK signaling pathway may be involved in its mechanism.
- Subjects :
- Blotting, Western
Carcinoma, Squamous Cell genetics
Carcinoma, Squamous Cell metabolism
Carcinoma, Squamous Cell pathology
Caveolin 1 genetics
Caveolin 1 metabolism
Cell Cycle
Cell Line, Tumor
Cell Survival
Flow Cytometry
Genetic Vectors chemistry
Genetic Vectors genetics
Humans
Laryngeal Neoplasms genetics
Laryngeal Neoplasms metabolism
Laryngeal Neoplasms pathology
Lipids chemistry
Microscopy, Confocal
Mitogen-Activated Protein Kinase 3 metabolism
Phosphorylation
Polymerase Chain Reaction methods
Signal Transduction physiology
Transfection methods
Apoptosis
Caveolin 1 physiology
Cell Proliferation
ErbB Receptors metabolism
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0253-3766
- Volume :
- 29
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Zhonghua zhong liu za zhi [Chinese journal of oncology]
- Publication Type :
- Academic Journal
- Accession number :
- 17892125