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Mechanisms in experimental venous valve failure and their modification by Daflon 500 mg.
- Source :
-
European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery [Eur J Vasc Endovasc Surg] 2008 Jan; Vol. 35 (1), pp. 102-10. Date of Electronic Publication: 2007 Sep 24. - Publication Year :
- 2008
-
Abstract
- Objectives: To characterize the acute response of the vein wall to venous hypertension and associated altered fluid shear stress and to test the effect of micronized purified flavonoid fraction (MPFF, Daflon 500), on this response.<br />Material and Methods: A femoral arteriovenous fistula was created in Wistar rats (n=48). A cohort of 24 rats received oral treatment with MPFF (100 mg/kg/day body weight), 24 rats underwent the arteriovenous fistula procedure and received no treatment. At days 1, 7 and 21 the animals (n=8 at each time point) were killed. Experimental parameters measured included limb circumference, blood flow at the sapheno-femoral junction, leukocyte infiltration and gelatinase activity (matrix metalloproteinase, MMP).<br />Results: The acute rise in venous hypertension was accompanied by limb edema and venous reflux together with an eventual loss of valve leaflets in the saphenous vein. There was an increase in granulocyte and macrophage infiltration into the venous wall and the surrounding tissue, and a lesser increase in T- and B-lymphocyte infiltration. These changes were accompanied by a local increase in the proteolytic enzymes, MMP-2 and MMP-9. Administration of MPFF reduced the edema and lessened the venous reflux produced by the acute arteriovenous fistula. Decreased levels of granulocyte and macrophage infiltration into the valves were also observed compared with untreated animals.<br />Conclusions: Venous hypertension caused by an arteriovenous fistula resulted in the development of venous reflux and an inflammatory reaction in venous valves culminating in their destruction. MPFF was able to delay the development of reflux and suppress damage to the valve structures in this rat model of venous hypertension.
- Subjects :
- Animals
Arteriovenous Shunt, Surgical
Blood Flow Velocity
Cardiovascular Agents therapeutic use
Chemotaxis, Leukocyte drug effects
Diosmin therapeutic use
Disease Models, Animal
Edema etiology
Edema physiopathology
Edema prevention & control
Femoral Artery surgery
Femoral Vein enzymology
Femoral Vein pathology
Femoral Vein physiopathology
Femoral Vein surgery
Granulocytes drug effects
Granulocytes pathology
Lymphocytes drug effects
Lymphocytes pathology
Macrophages drug effects
Macrophages pathology
Male
Matrix Metalloproteinase 2 metabolism
Matrix Metalloproteinase 9 metabolism
Rats
Rats, Wistar
Regional Blood Flow
Saphenous Vein enzymology
Saphenous Vein pathology
Saphenous Vein physiopathology
Stress, Mechanical
Time Factors
Venous Insufficiency complications
Venous Insufficiency enzymology
Venous Insufficiency pathology
Venous Insufficiency physiopathology
Cardiovascular Agents pharmacology
Diosmin pharmacology
Femoral Vein drug effects
Saphenous Vein drug effects
Venous Insufficiency drug therapy
Venous Pressure drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2165
- Volume :
- 35
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery
- Publication Type :
- Academic Journal
- Accession number :
- 17890112
- Full Text :
- https://doi.org/10.1016/j.ejvs.2007.08.011