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Different production of soluble HLA-G antigens by peripheral blood mononuclear cells in ulcerative colitis and Crohn's disease: a noninvasive diagnostic tool?
- Source :
-
Inflammatory bowel diseases [Inflamm Bowel Dis] 2008 Jan; Vol. 14 (1), pp. 100-5. - Publication Year :
- 2008
-
Abstract
- Background: HLA-G antigens are nonclassical major histocompatibility complex (MHC) class I molecules characterized by tolerogenic and antiinflammatory properties. Recently, a different expression of HLA-G antigens has been observed between intestinal biopsies of ulcerative colitis (UC) and Crohn's disease (CD) patients. These data suggested a functional role for HLA-G molecules in the diseases and proposed the HLA-G modulation as a marker for the diagnosis of UC and CD. The soluble HLA-G antigens (sHLA-G) are circulating molecules mainly produced by activated peripheral blood CD14+ monocytes.<br />Methods: We tested, by specific enzyme-linked immunosorbent assay (ELISA), the sHLA-G molecule levels in the supernatants of unstimulated and bacterial lipopolysaccharide (LPS)-stimulated cultures of peripheral blood mononuclear cells (PBMC) from 30 healthy subjects, 10 CD, and 18 UC patients. The data were not influenced by treatment or disease activity.<br />Results: The results confirmed a different sHLA-G expression between the diseases, with a spontaneous secretion of sHLA-G in CD patients but not in UC and healthy subjects. Moreover, a lack of sHLA-G antigens has been reported in UC patient cultures after LPS activation but not in healthy subjects and CD patients. The defective sHLA-G production was related to an impaired IL-10 secretion in UC but not in CD.<br />Conclusions: Overall, these results confirm the presence of a different biological characteristic between CD and UC patients and suggest sHLA-G production by PBMC as a noninvasive diagnostic tool in the early phases of the diseases.
- Subjects :
- Adult
Aged
Aged, 80 and over
Biomarkers
Blood Chemical Analysis
Enzyme-Linked Immunosorbent Assay methods
Female
HLA-G Antigens
Humans
Interleukin-10 metabolism
Male
Middle Aged
Colitis, Ulcerative diagnosis
Colitis, Ulcerative immunology
Crohn Disease diagnosis
Crohn Disease immunology
HLA Antigens biosynthesis
Histocompatibility Antigens Class I biosynthesis
Leukocytes, Mononuclear immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1078-0998
- Volume :
- 14
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Inflammatory bowel diseases
- Publication Type :
- Academic Journal
- Accession number :
- 17886287
- Full Text :
- https://doi.org/10.1002/ibd.20281