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Up-regulation of urinary UPIII mRNA levels in vesicoureteral reflux patients: potential application as a screening test for vesicoureteral reflux.
- Source :
-
International journal of urology : official journal of the Japanese Urological Association [Int J Urol] 2007 Oct; Vol. 14 (10), pp. 918-23. - Publication Year :
- 2007
-
Abstract
- Objectives: Vesicoureteral reflux (VUR) is the most common congenital urinary tract anomaly. This disease can pose a major threat to the kidneys as twenty percent of patients with endstage renal disease are reported to have VUR. Although genetic studies for uroplakin III (UPIII) have been reported recently, no study has focused on UPIII gene expression in VUR patients. We describe here the up-regulation of UPIII mRNA in exfoliated urinary cells from primary VUR patients.<br />Methods: A real-time RT-PCR for UPIII mRNA was performed on exfoliated urothelial cells from 18 primary VUR and 38 control samples. UPIII mRNA copies were calculated for each sample. The statistical differences were assessed by the Mann-Whitney U test. Receiver operator characteristic curves were constructed for analysis of the diagnostic values.<br />Results: UPIII mRNA was found to be up-regulated to a greater extent in VUR than in control exfoliated urinary cells (mean +/- SE: 497.0 +/- 178.5 copies vs. 69.0 +/- 10.0 copies, respectively, P < 0.001). In evaluating the measurement of urinary UPIII mRNA as a screening test for VUR, the sensitivity was 77.8% and the specificity was 76.3% by the best diagnostic cutoff point.<br />Conclusions: This is the first report demonstrating up-regulation of UPIII in mRNA levels in VUR patients. We submit that the quantitative measurement of urinary UPIII mRNA has a potential of developing into the first non-invasive screening test for VUR.
- Subjects :
- Adolescent
Adult
Aged
Child
Child, Preschool
Female
Humans
Infant
Infant, Newborn
Male
Membrane Glycoproteins biosynthesis
Middle Aged
RNA, Messenger biosynthesis
Uroplakin III
Urothelium pathology
Vesico-Ureteral Reflux metabolism
Vesico-Ureteral Reflux pathology
Mass Screening
Membrane Glycoproteins genetics
RNA, Messenger urine
Up-Regulation genetics
Vesico-Ureteral Reflux diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 0919-8172
- Volume :
- 14
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- International journal of urology : official journal of the Japanese Urological Association
- Publication Type :
- Academic Journal
- Accession number :
- 17880289
- Full Text :
- https://doi.org/10.1111/j.1442-2042.2007.01861.x