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Patterns of resistance to beta-lactams and characterization of beta-lactamases in Escherichia coli isolates from children in Tunisia.
- Source :
-
Journal of chemotherapy (Florence, Italy) [J Chemother] 2007 Aug; Vol. 19 (4), pp. 382-7. - Publication Year :
- 2007
-
Abstract
- Seventy-six (75.2%) of 101 Escherichia coli isolates, collected over a 2-month period in 2002 at the Children's Hospital of Tunis (Tunisia), were resistant to at least one beta-lactam. They produced beta-lactamases which were further characterized by spectrophotometry, isoelectric focusing (IEF) and PCR. Seventy-five isolates had a pI 5.4 beta-lactamase and one isolate expressed a pI 5.6 beta-lactamase. These beta-lactamases were active against penicillins and cephalothin. Nineteen of 76 isolates were resistant to ticarcillin-clavulanic acid combination. The bla(TEM) gene was detected in 71 isolates, all expressed a pI 5.4 beta-lactamase that was assumed to be TEM-1 or inhibitor-resistant TEM (IRT). Thirty-eight of 76 isolates showed one weak band on IEF with pIs ranging from 8.2 to >9, suggesting low level expression of the chromosomal AmpC beta-lactamase. Five of 76 isolates produced extended-spectrum beta-lactamases (ESBLs), with a basic pI of 7.9 or 8.7, active on penicillins, extended-spectrum cephalosporins, but not on cefoxitin. bla(SHV) genes were detected in three isolates producing pI 7.9 ESBLs but not in two isolates expressing pI 8.7 ESBLs. These latter showed strong cefotaxime-hydrolyzing activities. Hence, they might be CTX-M-type ESBLs.
- Subjects :
- Anti-Bacterial Agents metabolism
Bacterial Proteins metabolism
Cephalothin metabolism
Cephalothin pharmacology
Child
Escherichia coli enzymology
Escherichia coli Infections microbiology
Humans
Hydrogen-Ion Concentration
Microbial Sensitivity Tests
Penicillins metabolism
Penicillins pharmacology
Tunisia
beta-Lactamases metabolism
beta-Lactams metabolism
Anti-Bacterial Agents pharmacology
Bacterial Proteins chemistry
Escherichia coli drug effects
beta-Lactam Resistance
beta-Lactamases chemistry
beta-Lactams pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1120-009X
- Volume :
- 19
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of chemotherapy (Florence, Italy)
- Publication Type :
- Academic Journal
- Accession number :
- 17855181
- Full Text :
- https://doi.org/10.1179/joc.2007.19.4.382