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Inhibitory effect of dietary n-3 polyunsaturated fatty acids to intestinal IL-15 expression is associated with reduction of TCRalphabeta+CD8alpha+CD8beta-intestinal intraepithelial lymphocytes.
- Source :
-
The Journal of nutritional biochemistry [J Nutr Biochem] 2008 Jul; Vol. 19 (7), pp. 475-81. Date of Electronic Publication: 2007 Sep 12. - Publication Year :
- 2008
-
Abstract
- Intestinal intraepithelial lymphocytes (IELs) and their cytokines play an important role in the regulation of gut immune response and take part in gut immune barrier function. n-3 polyunsaturated fatty acid (PUFA) is an immunoregulator that has been shown to influence the process of gut inflammation. Interleukin (IL)-15 is a T-cell growth factor that has been shown to influence the differentiation of IEL. The aim of this study was to analyze the effects of dietary n-3 PUFA on IEL. IEL phenotype and cytokine (TNF-alpha, IFN-gamma, IL-4, IL-10 and TGF-beta1) profile were measured by FACS and real-time RT-PCR in healthy adult rats fed with fish oil diet for 90 days. Rats fed with corn oil diet served as controls. Intestinal IL-15 expression was measured by immunohistochemistry and real-time RT-PCR. The results demonstrated a decrease of intestinal IL-15 expression in the fish oil group. Associated with this deduction, n-3 PUFA significantly decreased the proportion of TCRalphabeta+CD8alpha+CD8beta- cells and IEL-derived TNF-alpha, IFN-gamma, IL-4 and IL-10. In conclusion, n-3 PUFA could inhibit intestinal mucosal expression of IL-15 and may influence phenotype and function of IEL through this mechanism.
- Subjects :
- Animals
Cytokines biosynthesis
Fatty Acids, Omega-3 administration & dosage
Intestinal Mucosa cytology
Lymphocytes drug effects
Male
Rats
Rats, Inbred Lew
CD8 Antigens physiology
Fatty Acids, Omega-3 pharmacology
Interleukin-15 biosynthesis
Lymphocytes immunology
Receptors, Antigen, T-Cell, alpha-beta physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0955-2863
- Volume :
- 19
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- The Journal of nutritional biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 17855067
- Full Text :
- https://doi.org/10.1016/j.jnutbio.2007.06.008