Back to Search
Start Over
Variability in the carbazole assay for N-desulfonated/N-acylated heparin derivatives.
- Source :
-
Carbohydrate research [Carbohydr Res] 2007 Dec 10; Vol. 342 (17), pp. 2664-9. Date of Electronic Publication: 2007 Aug 23. - Publication Year :
- 2007
-
Abstract
- The carbazole assay is commonly employed to quantify heparin and other uronic acid-containing polysaccharides. Heparin-derived standard curves are often employed to quantify solutions of various natural and unnatural heparin structures that have different levels of sulfate substitution, different levels of N-sulfo and N-acetyl groups, and other structural changes as a consequence of reducing molecular weight. Recent studies in our laboratory have focused on chemically modified heparin derivatives comprised of structurally diverse N-acyl moieties substituted into heparin in place of N-sulfo groups. We report here that although differing degrees of 2-N-sulfo-, 2-N-acetyl- or 2-amino-d-glucosamine residues within heparin do not affect signal intensity in the carbazole assay, replacing N-sulfo groups in heparin with structurally diverse N-acyl moieties affords products that display significant variation in the assay. The structure of different N-acyl groups, and to a lesser extent the degree of N-acylation by individual N-acyl groups, is shown to variably alter signal intensity in the carbazole assay even though content and structure of uronic acid residues is unaltered.
Details
- Language :
- English
- ISSN :
- 0008-6215
- Volume :
- 342
- Issue :
- 17
- Database :
- MEDLINE
- Journal :
- Carbohydrate research
- Publication Type :
- Academic Journal
- Accession number :
- 17850773
- Full Text :
- https://doi.org/10.1016/j.carres.2007.08.004