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Effects of potassium on the anion and cation contents of primary cultures of mouse astrocytes and neurons.
- Source :
-
Neurochemical research [Neurochem Res] 1991 Dec; Vol. 16 (12), pp. 1275-83. - Publication Year :
- 1991
-
Abstract
- In astrocytes, as [K+]o was increased from 1.2 to 10 mM, [K+]i and [Cl-]i were increased, whereas [Na+]i was decreased. As [K+]o was increased from 10 to 60 mM, intracellular concentration of these three ions showed no significant change. When [K+]o was increased from 60 to 122 mM, an increase in [K+]i and [Cl-]i and a decrease in [Na+]i were observed. In neurons, as [K+]o was increased from 1.2 to 2.8 mM, [Na+]i and [Cl-]i were decreased, whereas [K+]i was increased. As [K+]o was increased from 2.8 to 30 mM, [K+]i, [Na+]i and [Cl-]i showed no significant change. When [K+]o was increased from 30 to 122 mM, [K+]i and [Cl-]i were increased, whereas [Na+]i was decreased. In astrocytes, pHi increased when [K+]o was increased. In neurons, there was a biphasic change in pHi. In lower [K+]o (1.2-2.8 mM) pHi decreased as [K+]o increased, whereas in higher [K+]o (2.8-122 mM) pHi was directly related to [K+]o. In both astrocytes and neurons, changes in [K+]o did not affect the extracellular water content, whereas the intracellular water content increased as the [K+]o increased. Transmembrane potential (Em) as measured with Tl-204 was inversely related to [K+]o between 1.2 and 90 mM, a ten-fold increase in [K+]o depolarized the astrocytes by about 56 mV and the neurons about 52 mV. The Em values measured with Tl-204 were close to the potassium equilibrium potential (Ek) except those in neurons at lower [K+]o. However, they were not equal to the chloride equilibrium potential (ECl) at [K+]o lower than 30 mM in both astrocytes and neurons.(ABSTRACT TRUNCATED AT 250 WORDS)
- Subjects :
- Animals
Anions
Astrocytes drug effects
Cations
Cells, Cultured
Dose-Response Relationship, Drug
Embryo, Mammalian
Hydrogen-Ion Concentration
Membrane Potentials drug effects
Mice
Nerve Tissue Proteins metabolism
Neurons drug effects
Potassium administration & dosage
Water metabolism
Astrocytes metabolism
Chlorides metabolism
Neurons metabolism
Potassium metabolism
Potassium pharmacology
Sodium metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0364-3190
- Volume :
- 16
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Neurochemical research
- Publication Type :
- Academic Journal
- Accession number :
- 1784325
- Full Text :
- https://doi.org/10.1007/BF00966658