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Catecholaminergic neurotransmitters regulate migration and repopulation of immature human CD34+ cells through Wnt signaling.
- Source :
-
Nature immunology [Nat Immunol] 2007 Oct; Vol. 8 (10), pp. 1123-31. Date of Electronic Publication: 2007 Sep 09. - Publication Year :
- 2007
-
Abstract
- Catecholamines are important regulators of homeostasis, yet their functions in hematopoiesis are poorly understood. Here we report that immature human CD34+ cells dynamically expressed dopamine and beta2-adrenergic receptors, with higher expression in the primitive CD34+CD38(lo) population. The myeloid cytokines G-CSF and GM-CSF upregulated neuronal receptor expression on immature CD34+ cells. Treatment with neurotransmitters increased the motility, proliferation and colony formation of human progenitor cells, correlating with increased polarity, expression of the metalloproteinase MT1-MMP and activity of the metalloproteinase MMP-2. Treatment with catecholamines enhanced human CD34+ cell engraftment of NOD-SCID mice through Wnt signaling activation and increased cell mobilization and bone marrow Sca-1+c-Kit+Lin- cell numbers. Our results identify new functions for neurotransmitters and myeloid cytokines in the direct regulation of human and mouse progenitor cell migration and development.
- Subjects :
- Animals
Cell Movement drug effects
Cell Proliferation drug effects
Dopamine pharmacology
Dopamine Agonists pharmacology
Hematopoietic Stem Cells cytology
Humans
Mice
Mice, Inbred C57BL
Norepinephrine pharmacology
Receptors, Dopamine analysis
Antigens, CD34 analysis
Catecholamines pharmacology
Hematopoietic Stem Cells drug effects
Neurotransmitter Agents pharmacology
Signal Transduction physiology
Wnt Proteins physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1529-2908
- Volume :
- 8
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Nature immunology
- Publication Type :
- Academic Journal
- Accession number :
- 17828268
- Full Text :
- https://doi.org/10.1038/ni1509