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Rapid detection and quantification of Chikungunya virus by a one-step reverse transcription polymerase chain reaction real-time assay.
- Source :
-
The American journal of tropical medicine and hygiene [Am J Trop Med Hyg] 2007 Sep; Vol. 77 (3), pp. 521-4. - Publication Year :
- 2007
-
Abstract
- Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus. A large outbreak of CHIKV disease occurred in 2005 in the Indian Ocean Islands. Many cases have been imported in European countries. Laboratory confirmation of suspected cases is mandatory for control measures during an outbreak. We report a novel, real-time, reverse transcription-polymerase chain reaction (RT-PCR) for the nonstructural protein 1 region that can quantify a wide range of viral RNA concentrations. This assay was validated by in vitro experiments in which interferon-alpha, a well-known virus inhibitor, showed a dose-dependent inhibition of virus replication on Vero cells that was assessed by viral infectivity and viral RNA production. This new real-time RT-PCR was used to measure viral load in serum samples from cases recently imported to Italy, and may be a useful tool in rapid detection of CHIKV and monitoring the extent of viral replication in patients.
- Subjects :
- Adult
Alphavirus Infections blood
Alphavirus Infections virology
Base Sequence
Female
Humans
Male
Middle Aged
RNA, Viral analysis
RNA, Viral genetics
Sensitivity and Specificity
Viral Load
Chikungunya virus genetics
Chikungunya virus isolation & purification
Reverse Transcriptase Polymerase Chain Reaction methods
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9637
- Volume :
- 77
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The American journal of tropical medicine and hygiene
- Publication Type :
- Academic Journal
- Accession number :
- 17827371